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1、中国水稻科学(ChinJRiceSci),２０１６,３０(１):１０－１６http://www．ricesci．cn１０DOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１３０水稻无选择标记Xa２１转基因系CX８６２１的获得与遗传分析夏志辉１,２,＃刘鹏程１,＃高利芬１刘栋峰１姜丽１江光怀１郭乐群１翟文学１,∗(１中国科学院遗传与发育生物学研究所,北京１００１０１;２海南省热带生物资源可持续利用重点实验室/海南大学农学院,海口５７０２２８;＃共同第一作者;∗通讯联系人,EＧmail:wxzhai＠genetics．ac．cn)GenerationandGeneticAnalysis
2、ofXa２１TransgenicRiceLineCX８６２１WithoutSelectableMarkersXIAZhiＧhui１,２,＃,LIUPengＧcheng１,＃,GAOLiＧfen１,LIUDongＧfeng１,JIANGLi１,JIANGGuangＧhuai１,GUOLeＧqun１,ZHAIWenＧxue１,∗(１InstituteofGeneticsandDevelopmentalBiology,ChineseAcademyofSciences,Beijing１００１０１,China;２HainanKeyLaboratoryforSustainableUtilizationof
3、TropicalBioresources/CollegeofAgriculture,HainanUniversity,Haikou５７０２２８,China;＃Theseauthorscontributedequallytothiswork;∗Correspondingauthor,EＧmail:wxzhai＠genetics．acc．n)XIAZhihui,LIUPengcheng,GAOLifen,etal．GenerationandgeneticanalysisofXa２１transgenicricelineCX８６２１withoutselectablemarkers．ChinJRiceS
4、ci,２０１６,３０(１):１０Ｇ１６．Abstract:SelectablemarkerＧfreeandstableinheritancearethebasicrequirementsforsafeapplicationoftransgeniccrops．FollowingtheregulationsapprovedbytheNationalCommitteeforTransgenicBiosafety,thewidespectrumbacterialblightresistancegeneXa２１wastransferredintoawidelyusedrestorerlineMinghu
5、i８６byusingadoublerightＧbordersTＧDNAvectorthroughAgrobacteriumtumefaciensＧmediatedsystem．ThetransgeneXa２１wasconfirmedbyresistanceidentification,PCRanalysisandSouthernblotintheprogenies．ThesingleＧcopyhomozygoustransgeniclineCX８６２１withoutselectablemarkerandvectorbackbonesequenceswasobtainedfromT３genera
6、tion．ThetransgenicrestorerlineCX８６２１hasstablebacterialblightresistancewithyearsofobservationandidentification．Uptonow,CX８６２１hasselfＧfertilizedto１６thgenerationwithstableinheritanceoftransgeneXa２１andpassedthebioＧsafetyevaluationofsmallandlargescaleoffieldtests(environmentalreleaseandproductivetest)．Ke
7、ywords:transgenicrice;bacterialblight;Xa２１;selectablemakerＧfree;stableinheritance夏志辉,刘鹏程,高利芬,等．水稻无选择标记Xa２１转基因系CX８６２１的获得与遗传分析．中国水稻科学,２０１６,３０(１):１０Ｇ１６．摘要:无选择标记和转基因稳定遗传是转基因作物安全应用的基本要求.按照我国转基因作物安全评价指南,利用双
