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时间:2019-05-19
《光质对番茄幼苗碳氮代谢及相关酶活性的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、园艺学报,2016,43(1):80–88.ActaHorticulturaeSinica80doi:10.16420/j.issn.0513-353x.2015-0691;http://www.ahs.ac.cn光质对番茄幼苗碳氮代谢及相关酶活性的影响11,2,*1,21,31,31,2孙娜,魏珉,李岩,王秀峰,杨凤娟,史庆华123(山东农业大学园艺科学与工程学院,农业部黄淮海设施农业工程科学观测实验站,作物生物学国家重点实验室,山东泰安271018)摘要:采用LED光源,研究了白光(对照)、红光、蓝光、紫光和红蓝(3︰1)组合光对番茄幼苗生长、碳
2、氮代谢及相关酶活性的影响。结果表明:与白光相比,紫光明显抑制幼苗生长,红光抑制幼苗,尤其是其地下部的生长,红蓝组合光下幼苗干物质积累量及壮苗指数最高;在紫光下叶片净光合速率(Pn)、RuBP羧化酶活性、总糖和淀粉含量最低,红蓝组合光下最高;红光和紫光下转化酶的活性较高;红、蓝、紫、红蓝组合光均显著提高蔗糖合成酶(SS)活性,以红蓝组合光更明显,但红、蓝、紫光显著降低蔗糖磷酸合成酶(SPS)活性;红光显著降低了硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)活性,蓝、紫、红蓝组合光则显著提高了GS、GOGAT活性以及叶片游离氨基酸
3、和可溶性蛋白含量。总之,红蓝组合光有利于番茄幼苗生长,促进碳同化及总糖和淀粉积累,提高氮代谢相关酶活性及可溶性蛋白和游离氨基酸含量。关键词:番茄;光质;碳代谢;氮代谢;生长中图分类号:S641.2文献标志码:A文章编号:0513-353X(2016)01-0080-09EffectsofLightQualityonCarbonandNitrogenMetabolismandEnzymeActivitiesinTomatoSeedlings11,2,*1,21,31,31,2SUNNa,WEIMin,LIYan,WANGXiu-feng,YANGFen
4、g-juan,andSHIQing-hua12(CollegeofHorticultureScienceandEngineering,ShandongAgriculturalUniversity,ScientificObservingandExperimentalStationofEnvironmentControlledAgriculturalEngineeringinHuang-Huai-HaiRegion,Ministryof3Agriculture,StateKeyLaboratoryofCropBiology,Tai’an,Shandong
5、271018,China)Abstract:Theeffectsofdifferentlightquality[(white,red,blue,purpleandred-blue(3︰1)]ongrowthandcarbon-nitrogenmetabolismintomatoseedlingswereinvestigatedbyusingLEDlightinginagrowthchamber.Theresultsshowedthat:Comparedwithwhitelight,purplelightinhibitedobviouslythegro
6、wthofseedlings,andredlightsuppressedthegrowthofroots,whilered-bluelightledtohighestdrymatterweightandseedlingindex;Pn,RuBPcarboxylaseactivities,andcontentsofsugarandstarchinleaveswerelowestinpurplelight,however,highestinred-bluelight.Theactivitiesofinvertaseswerehigherinbothred
7、andpurplelighting.Red,blue,purpleandred-bluelightboostedtheactivitiesofsucrosesynthase,however,red,blue,andpurplelightreducedtheactivitiesofsucrosephosphatesynthase(SPS).Theactivitiesofnitratereductase(NR),glutaminesynthetase(GS)andglutamatesynthetase(GOGAT)收稿日期:2015–11–12;修回日期
8、:2016–01–12基金项目:‘十二五’国家科技支撑计划课题(2012BAD11B01;2014BAD05
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