活体动物基因表达的分子影像学研究方法

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1、活体动物基因表达的分子影像学研究方法徐宇虹August2008,Hangzhou基因表达的时空特征常规的mRNA的检测蛋白产物的检测-->断裂的时间信息,缺失的空间信息分子影像学方法!什么是分子影像Molecularimaging---Imaging“molecularevents”/”biologicalprocesses”ina“systems”setting无创-->连续多次观查-->时间序列信息影像-->空间信息ImagingmodalitiesCT3Dobjectsetof2DprojectionsPrincipleofelectrontomograph

2、yTheprincipleoftomographicreconstruction:CollectmultipleprojectionsOfa3-DobjectContrastMechanismNonionicmonomerFromR.Older,:internettutorialIodinatedContrastCompoundsCompound[Iodine]mg/mLmOsm/kgIonicmonomerupto4001400-2100Ionicdimer320600Nonionicmonoupto350600-800Nonionicdimer320290Hig

3、hResolutionSpiralCTVisualizingvertebralforamenaBudingeretal.,LBNLandNCSAMRIContrastMechanismGadolinium-BasedContrastParamagneticagentDecreasesT1relaxationtimesToxicinfreestateGadodiamide(Omniscan)FenanoparticlescontrastingagentsThesusceptibilityeffectofirongiveshighcontrastthatisobserv

4、ableonT2-weightedinMRIUSPIOaredexran-coatedironoxidenanoparticlesthatareinternalizedintomononuclearphagocyticcellsLymphocyteswithoutcontrastingagentLymphocyteswithcontrastingagentUSreal-timeimaging;relativelyshortandefficientimagingprotocols,comparedwithMRIornuclearimaging;noninvasiven

5、ess(intheabsenceofcontrast)tominimizepatientdiscomfort;lowoperatingcosts;largeinstalledequipmentbase;andcomplementarytherapeuticandimagingapplications.UltrasoundContrastAgentsIMAGENT:perflexane(stablegas)lipidmicrospheresDonotgivetopatientswithcardiacshunts14%reportedAE(compareto11%wit

6、hsaline):headache,nauseamostcommonOPTISON:humanalbuminmicrosphereswithoctafluoropropaneContraindicatedifhypersensitivitytobloodproducts17%reportedAE:headache,nausea,flushing,dizzinessPETContrastMechanismOpticalcontrastmechanismFor‘Transparent’AnimalsOnlyBiologyAnatomyMetabolismReceptor

7、bindingGeneexpressionCelltraffickingCelldeathModalityX-rayCTSPECTPETMRIBioluminescenceFluorescenceMajordiseasesCardiovasculardiseaseCancerDepressionArthritisAlzheimer’sDiseaseParkinson’sdiseaseMolecularimaging(geneexpressionimaging)Whatismolecularresolution?Imaginggeneexpressionprod