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ID:36806448
大小:1.12 MB
页数:37页
时间:2019-05-15
《黄体中期子宫内膜类型与白血病抑制因子的相关性研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、黄体中期子宫内膜类型与白血病抑制因子的相关性研究中文摘要本研究对64例不孕患者在黄体中期行宫腔镜检查,根据黄体中期宫腔镜下子宫内膜腺体开口和内膜血管网形态学表现将内膜分为两类,I类:腺管开口小,或内膜血管网呈点状或片状。II类:腺管开口极度扩张呈指环状,且内膜血管网呈网状分布。在宫腔镜检查后,收集两类患者的宫腔冲洗液和进行阴道超声测定子宫内膜厚度。采用酶联免疫吸附实验(ELISA)双抗体夹心法检测宫腔冲洗液中LIF的浓度。研究发现,1)在I类内膜中宫腔冲洗液LIF浓度为14.38±15.16pg/ml,II类内膜为61.70-t-44.13pg/ml。与增生期宫腔液中LIF浓度
2、儿.02±11.07pg/ml相比,黄体中期II类内膜中宫腔冲洗液LIF浓度较增生期内膜(对照组)明显升高,而I类内膜中无明显变化。2)在I类内膜中,其子宫内膜厚度为10.2l±2.32mm,在II类内膜中,其子宫内膜厚度为10.36±2.29mm,两类内膜在黄体中期阴超下子宫内膜厚度比较,无显著性差异(P>0.05)。3)在I类内膜中有原发不孕患者20例,继发不孕患者9例;在II类内膜中原发不孕患者10例,继发不孕患者25例;两类内膜中原发不孕和继发不孕患者的分布有显著性差异(P3、。以月经周期规则的停经40一49天、经B超证实为早期宫内妊娠的人工流产蜕膜组织为对照组。提取上述组织中的总RNA,经DNA酶处理后,用特异性引物行RT—PCR反应,扩增出特异性片断,产物经1%的琼脂糖电泳后,用TanonGIS凝胶图像处理系统扫描、分析LIFmRNA在内膜中的表达水平。I组黄体中期子宫内膜中LIFmRNA的相对转录丰度值为0.93i0.02,II组为l-55i0.08,人工流产组蜕膜中(对照组)LIFmRNA的表达水平为1.65±0.18。I组黄体中期LIFmRNA的表达水平比对照组显著下降(P4、性差异(P>O.05)。结论:通过对不孕妇女在黄体中期进行宫腔镜检查和宫腔液LIF浓度检测,具有操作简单,病人耐受性好的优点,对评估子宫内膜的容受性有一定意义。黄体中期子宫内膜LIF的表达与此期子宫内膜形态的发育有一定的相关性。单纯通过测量子宫内膜厚度来评估子宫内膜对胚胎的容受性是不可靠的。关键词黄体中期;着床;子宫内膜容受性;官腔镜;LIF;阴道超声检查2TheRelationshipoftheMid—secretaryEndometriuminHysteroscopytoLeukaemiaInhibitoryFactorintheUterusAbstract64infert5、ilewomenunderwenttheobservationofhysteroscopy,measurementofendometrialthicknessbytransvaginalultrasoundandconectionofuterineflushingforLIFassayinthemid-secretaryphase.Theywereclassifiedendoscopicallyaccordingtotheappearanceofboththeglandularopeningsandthebloodvesselsontheendometriumsurfaceas6、eitherGroupIorGroupII.GroupI:dot-and/orpunctate—typedglandularopeningsand/orbadvascularity.GroupII:ring—typeglandularopeningsandwell-developedvascularnetworks.AnELISAkitspecificforhumanLIFwasusedforLIFassay.ThemedianLIFconcentrationmeasuredinGroup1was14.375±15.155pg/m1.Itwas61.696±44.127pg/m7、linGroupII,and11.02±11.07pg/mlintheendometriumduringtheproliferativephaseofthecycle.ThedifferenceofLIFconcentrationwasstatisticallysignificant(P<0.05)betweenGroupIandtheproliferativeendometrium.ButtherewasnosignificantdifferencebetweenGroupIIandthe
3、。以月经周期规则的停经40一49天、经B超证实为早期宫内妊娠的人工流产蜕膜组织为对照组。提取上述组织中的总RNA,经DNA酶处理后,用特异性引物行RT—PCR反应,扩增出特异性片断,产物经1%的琼脂糖电泳后,用TanonGIS凝胶图像处理系统扫描、分析LIFmRNA在内膜中的表达水平。I组黄体中期子宫内膜中LIFmRNA的相对转录丰度值为0.93i0.02,II组为l-55i0.08,人工流产组蜕膜中(对照组)LIFmRNA的表达水平为1.65±0.18。I组黄体中期LIFmRNA的表达水平比对照组显著下降(P4、性差异(P>O.05)。结论:通过对不孕妇女在黄体中期进行宫腔镜检查和宫腔液LIF浓度检测,具有操作简单,病人耐受性好的优点,对评估子宫内膜的容受性有一定意义。黄体中期子宫内膜LIF的表达与此期子宫内膜形态的发育有一定的相关性。单纯通过测量子宫内膜厚度来评估子宫内膜对胚胎的容受性是不可靠的。关键词黄体中期;着床;子宫内膜容受性;官腔镜;LIF;阴道超声检查2TheRelationshipoftheMid—secretaryEndometriuminHysteroscopytoLeukaemiaInhibitoryFactorintheUterusAbstract64infert5、ilewomenunderwenttheobservationofhysteroscopy,measurementofendometrialthicknessbytransvaginalultrasoundandconectionofuterineflushingforLIFassayinthemid-secretaryphase.Theywereclassifiedendoscopicallyaccordingtotheappearanceofboththeglandularopeningsandthebloodvesselsontheendometriumsurfaceas6、eitherGroupIorGroupII.GroupI:dot-and/orpunctate—typedglandularopeningsand/orbadvascularity.GroupII:ring—typeglandularopeningsandwell-developedvascularnetworks.AnELISAkitspecificforhumanLIFwasusedforLIFassay.ThemedianLIFconcentrationmeasuredinGroup1was14.375±15.155pg/m1.Itwas61.696±44.127pg/m7、linGroupII,and11.02±11.07pg/mlintheendometriumduringtheproliferativephaseofthecycle.ThedifferenceofLIFconcentrationwasstatisticallysignificant(P<0.05)betweenGroupIandtheproliferativeendometrium.ButtherewasnosignificantdifferencebetweenGroupIIandthe
4、性差异(P>O.05)。结论:通过对不孕妇女在黄体中期进行宫腔镜检查和宫腔液LIF浓度检测,具有操作简单,病人耐受性好的优点,对评估子宫内膜的容受性有一定意义。黄体中期子宫内膜LIF的表达与此期子宫内膜形态的发育有一定的相关性。单纯通过测量子宫内膜厚度来评估子宫内膜对胚胎的容受性是不可靠的。关键词黄体中期;着床;子宫内膜容受性;官腔镜;LIF;阴道超声检查2TheRelationshipoftheMid—secretaryEndometriuminHysteroscopytoLeukaemiaInhibitoryFactorintheUterusAbstract64infert
5、ilewomenunderwenttheobservationofhysteroscopy,measurementofendometrialthicknessbytransvaginalultrasoundandconectionofuterineflushingforLIFassayinthemid-secretaryphase.Theywereclassifiedendoscopicallyaccordingtotheappearanceofboththeglandularopeningsandthebloodvesselsontheendometriumsurfaceas
6、eitherGroupIorGroupII.GroupI:dot-and/orpunctate—typedglandularopeningsand/orbadvascularity.GroupII:ring—typeglandularopeningsandwell-developedvascularnetworks.AnELISAkitspecificforhumanLIFwasusedforLIFassay.ThemedianLIFconcentrationmeasuredinGroup1was14.375±15.155pg/m1.Itwas61.696±44.127pg/m
7、linGroupII,and11.02±11.07pg/mlintheendometriumduringtheproliferativephaseofthecycle.ThedifferenceofLIFconcentrationwasstatisticallysignificant(P<0.05)betweenGroupIandtheproliferativeendometrium.ButtherewasnosignificantdifferencebetweenGroupIIandthe
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