山莨菪碱对肠缺血再灌注致多器官损伤的保护作用

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1、广西医科大学硕士学位论文山莨菪碱对肠缺血再灌注致多器官损伤的保护作用姓名：蒋卓汛申请学位级别：硕士专业：病理生理学指导教师：程建祥2003.5.1主要英文缩写缠星墓室垒憨生塞全签MODSmultipleorgandysfunctionsyndrome多器官功熊障碍综合征SIRSsystemicinflammatoryresponsesyndrome全身炎症反应I理tSoDMDAPMNTNFischemiareperfusioninjurysuperoxidedismutasemalOndiaIdehydepolymor

2、phonuelearneurtophilstumornecrosisfaetorN0nitricoxideMPOmyeloperoxidaseON00-peroxynitrite综会征缺斑霉灌注损挠超氧化物歧化酶丙二鹱中性粒细胞肿瘤坏死因子一氧化氮髓过氯纯豹酶过氧化亚磷酸阴离子3出莨蓉城对肠缺巍再灌注致多器宫按伤的保护作用摘要目的：探讨肠缺血樽灌浪引起肠外多器官损伤的作用及其机理，并观察山莨菪碱对损伤的保护作用。方法：用无创动脉夹夹闭大鼠肠系膜上动脉120min后，松夹再灌注2h、6h，建立肠缺血再灌注模型，于缺血前5

3、rain、缺血后60rain、稃灌涟前5rain分别殷静脉注射由莨菪碱(10mg／kg)。／l结栗：肠缺盎再灌注损伤后，_

4、l干、肾功熊的生纯指标舞蔫；器、瞥、蒡奉缓织中静MDA含豢升离，SOD活性降低；嚣手、膝缝织中的MPO活性增熬；囊滂NO、TNF．程浓度升麓；盘清NO与MDA食量变他呈难相关。出莨菪碱可缓解肠缺血再灌注对肠外器官的损伤，减少MDA的生成，增强SOD的清除能、／力，降低MPO活性，抑制NO、TNF．a浓度升籀。y结论：肠缺血再灌注可导致肠外多器官损伤，可能与肠道细菌、内蒜素移位激活单核．巨噬细胞系统

5、以及中性粒细胞，释放过量炎症介质，TNF。Or．、NO等，导致炎症失控的SIRS和缺血再灌注本身产生大量自由基有关，对多器官产生综合性损伤的结果。而山莨菪碱对肠缺血两灌注引起的多器官损伤有保护作用，可能与抑制翻由基损伤，减轻中性粒细胞聚集及活化，抑制过度炎痰反应及大羹NO释放有关。关键词：洳蓑碧甄；肠蛱盘争}森；多器富功il《磊综会征ProlectiveeffectsofanisodamineonmultipleorgandamageduringintestinalischemiareperfusionAbstraet

6、Objective：Toinvestigatetheprotectionofanisodamineduringintestinalischemiareperfusion(I／R)andtoexaminethemechanismbywhichthemultipleorganwasdamaged．Methods：Intestinalisehemiareperfusionwasinducedbyclampingsuperiormesentericarteryfor120rainperiodandthenreleasingar

7、terialclampingfor2hoursor6hours。Anisodaminewasadministeredintravenously5minutesbeforeisehemic、60minutesafterisehemicand5minutesbeforereperfusion．Results：Afterintestinalischemiareperfusioninjury，thebiochemistryincreasedwhichindicatedthatfunctionofliverandkidneywa

8、sdamaged．ThelevelofMDAwasgraduallyincreased，whiletheactivitiesofSODweregraduallydecreasedinliver、lungandkidneyhomogenate·ThelevelofMPOwasgraduallyincreasedinliverandlunghomogenate．TheserumNO、TNF-aconcentrationsafterreperfusionwerealsosignificantlyhigherthanthose

9、ofsham—operatedcontrolgroup．AclosecorrelationwasshownbetweenMDAcontentandNOcontentinserum．Furthermore，theresultsshowedthatpretreatmentwithanisodaminenotablyimprovedth