肉桂对枝枯病菌的抗性变异研究

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1、VariationinresistanceofCinnamomumcassiatoBotryodiplodia肠即云，.配Su叭M.乙(CollegeofForestry,SouthChinaAgriculturalUnivers呱Guangzhou510642)AbM曲mdTheuseofresistantvarietiesisasimple,economic.目efectivewayincontrollingplantdiseases.Butfirstthesourceofresistanceanditsmechanismshouldbed

2、eterminedsothat山edevelopmentofresistantvarietiescanproceed.FiveC.~treesatSouthChinaAgriculturalUniversitytreegardenpresumablywithdiferentoriginswereselectedfortheexperiment.Thebranchcutingsofthesetreeswereinoculatedwithmyceliumblocksof2BotryodiplodiatheobromaeisolatesL3andX,

3、,Areasofthebrowndiseasespotsweremeasuredandanalyzedafterinoculation.TheresultdemonstratedtheexistenceofsignificantvariationinresistancetoB.theobromaeblightdiseaseamongindividualC.cassiatrees.Thevariationbetween2fungalisolateswasalsosignificant.Theinteractionbetweenthetreesan

4、dpathogens,however,wasnotsignificant.Theseresultssuggestthatselectionforresistancecouldbeachievedfromindividualtreesinthispathosystemandtheresistanceoftreetothepathogenispossiblyhorizontal,sincetheexpressionoftheresistanceamongindividualtreeswasconsistentondiferentfungal右叭..

5、奋，电ThebarkextractofC.cassiawasobtainedinthisexperiment.叹however,hadnotbeenfoundtopossesanyinhibitoryefectonthe州hogengrowingonPDAplates.TheresultsuggeststheresistanceamongindividualC.cassiatreeswasnotduetotheirdiferenceinpre-infectionsubstancescontainedinthebark;otherresistan

6、cemechanismscouldbeinvolved.CallusinductionofCcassiatreewasinitiallyobtainedinthisexperiment.Butthesubculturewasnotsuccessful.Shoottips(1.0-1.3cminlength)from5yearsoldfieldtreeswasusedastheexplants.Murash呼andSkoog〔MS)mediums叩plementedwithdiferentconcentrationsof坑-benzyladeni

7、ne(BA)wasusedfor小.callusinduction.Inaseparateexperimentleafsegments(5X5mm)wasusedastheexplants.MSmediumwasalsousedbutsupplementedwithdiferentcombinationsofBAandI-t帅thaleneaceticacid(NAA).Intheshoot印experimentcallusinductionoccurredonalllevelsofBAmediaThealaiinducedweresmalli

8、nsizewithcoarse..云..andstagnatedafter4to5weeks.InLeafsegmentexperiment,all加