血小板衍生生长因子β在兔蛛网膜下腔出血后脑血管痉挛模型中的表达

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1、苏州大学硕士学位论文血小板衍生生长因子-β在兔蛛网膜下腔出血后脑血管痉挛模型中的表达姓名：崔红凯申请学位级别：硕士专业：影像医学与核医学指导教师：刘一之20070501血小扳衍生生长园子-B在兔蛛网膜下腔出血后脑血管痉挛模型中的表达英文摘要TheExpressionofPlatelet—DerivedGrowthFactor-pinCerebralVasospasmafterSubarachnoidHemorrhagewithaRabbitEndovascularPunctureModelAbstractObjective：Tostudytheexpressi

2、onandtimeCOHI'Seofplatelet-derivedgrowthfactor-pincerebralvasospasmaftersubaranhnoidhemorrhagewitharabbitendovascularpuncturemodel，andtodiscussthesignificance．MaterialandMethods：CerebralvasospasmmodelwasproducedafterSAHbYendovasculaxpuncturemethod．35NewZealandwhiterabbitswithhealtha

3、ndclean．TheSAIlgroupswel'edividedintoSCVerlgroups(including3h、12h、ld、2d、3d、7d、14d)，everygrouphasfiverabbits．Allrabbitsweretakenveinbloodbyeachtime．Thes哪mlevelsofPDGF—BB、】l舢detectedbymeansofe咀Zyrflelinkedimmunosorbentassay(ELISA)．Afterperfusion-fixed，sectionedsliceswereexaminedbyligh

4、tmicroscopytomea吼I聆luminalnarrowingandwallthicknessofposteriorcommunicatingarteryandbasfl盯artery．TheimmunohistochemistrytechniqueWasusedtostudyexpressofplatelet-derivedgrowthfactor-pinSmoothmusclecellwithbasilararteryandposteriorcommunicatingartery．Result：Amongall，brainsof45rabbits(

5、including35rabbitswithsubarachnoidhemorrhage)wereharvested．Compareingwithnormalgroupandnonoperationgroup，posteriorcommunicatingarteryandbasfl盯arteryshowedluminalnarrowingandwallthickness．EnhancedexpressionofPDGF—DproteinatCVSWass∞nat3h，anditsmarkedup—regulationwasnotedat3-7days，andt

6、hentheexpressionbecamedecreasedbutitremainedabovethecontrollevelsstill14daysafI盯cerebralvasospasm．Conclusion：Wedemonstratethatthismodelshowesbiphasicpatternstotheluminalnarrowing：acllteanddelayedconstrictionphase．TheexpressionofPDGF—p¨垒!：堡堑竺生苎里王：望垄墨生塑堕!些坐些生堕皇竺垄皇垡型主塑墨垄茎苎塑塞obviousdsea

7、ndthe陀glllafchangeintimec．，ourseincerebralvasospasmaftersubarachnoidhemorrhage．ItisimportantsignificanceinthecourseofoccurrenceanddevelopmentpathologyafI盯cerebralvasospasm．KeyWords：cerebralvasospasm；rabbit；subarachnoidhemorrhage；platelet-dedvedgrowthfactor-pIUWrittenby：CuiHongKaiSup

8、ervisedby：LiuYiZhi血