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1、770ActaPhysiologicaSinica,December25,2007,59(6):770-776http://www.actaps.com.cnResearchPaperSelectiverevealingofgapjunctioncurrentsinsingleinspiratorytrachealmotorneuronsCHENYong-Hua,HOULi-Li,WANGJi-Jiang*TheStateKeyLaboratoryofMedicalNeurobiology,FudanUniversityShanghaiM
2、edicalCollege,Shanghai200032,ChinaAbstract:Littleisknownabouthowgapjunctionsareinvolvedintherespiratory-relatedorothertypesofphysiologicalneuronalactivitysincephysiologicallyactivegapjunctioncurrents(GJCs)haveneverbeencharacterizedfromsinglerespiratory-relatedneuronsorfro
3、msingleneuronsofanyothertypes.InthepresentstudywehypothesizedthatGJCscouldbeselectivelyrevealedfromsingleneuronsbyeliminationoftransmembraneelectrochemicalgradientsinvoltagepatch-clamprecording,andthishypothesiswastestedinsingleinspiratorytrachealpreganglionicvagalmotorne
4、urons(I-TPVMs).TheresultsshowedthatGJCswereselectivelyrevealedinallI-TPVMswhenthetransmembraneelectrochemicalgradientswereeliminatedinvoltagepatch-clamprecording,andwererhythmicallyactivatedbycentralinspiratoryactivity.Therefore,thismethodmaybeusedasafastwaytodetectGJCswi
5、thinspontaneouslyactiveneuronalnetworks.Keywords:gapjunction;respiration;motorneurons;patch-clamptechniques电突触电流在单个吸气性气管运动神经元中的选择性显示陈咏华，侯丽丽，王继江*复旦大学上海医学院医学神经生物学国家重点实验室，上海200032摘要：在呼吸相关神经元或其它任何类型的神经元中，与生理性自发活动相对应的电突触电流(gapjunctioncurrents,GJCs)尚未在单个神经元中被记录到，因此电突触如何参与呼吸相关的或
6、其它类型的生理性活动，目前所知甚少。在本研究中，我们假设GJCs可在电压钳记录条件下通过消除跨膜电化学梯度在单个神经元实现选择性记录，并在单个吸气性气管迷走神经节前神经元(inspiratorytrachealpreganglionicvagalmotorneurons,I-TPVMs)进行验证。结果显示，用这种方法在所有I-TPVMs中均记录到GJCs，且这些神经元的GJCs可被节律性中枢吸气活动所激活。此法可用于快速探测具自发活动的神经元网络中的GJCs。关键词：电突触；呼吸；运动神经元；膜片钳技术中图分类号：R332.3；R338G
7、apjunctionsarebeingfoundinmoreandmoreareasortionsinrespiratorycontrol.Previousstudieshaveattemptedneuronalnetworksinthecentralnervoussystem[1].Intoobtaingapjunctioncurrents(GJCs)bypairedpatch-brainstemgapjunctionshavebeenfoundinrespiratoryclamprecordingoftworespiratory-re
8、latedneurons(dualrhythmogenesisneuronsinthepre-Botzingercomplex[2,3],patch)[3,5].Usingthismethod
