卵巢透明细胞癌基因表达谱的特征及其重要相关基因验证

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1、上海交通大学硕士学位论文卵巢透明细胞癌基因表达谱的特征及其重要相关基因验证姓名：缪懿申请学位级别：硕士专业：妇产科学指导教师：万小平20070501第二部分膜联蛋白IV及人组织激肽释放酶10在卵巢透明细胞癌中的特异性表达目的：验证膜联蛋白IV及人组织激肽释放酶10在卵巢透明细胞癌组织中的特异性表达。方法：利用定量PCR以及免疫组织化学对卵巢透明细胞癌组织中膜联蛋白IV及人组织激肽释放酶10的表达进行检测。结果：经实验证实，膜联蛋白IV在卵巢透明细胞癌组织中较浆液性癌组织存在表达上调，人组织激肽释放酶10在

2、卵巢透明细胞癌组织中较浆液性癌组织存在表达下调。结论：膜联蛋白IV及人组织激肽释放酶10可能成为卵巢透明细胞癌特异性的肿瘤标志物，为实现肿瘤基因治疗提供了分子依据以及新的靶标。关键词：卵巢透明细胞癌，膜联蛋白IV，人组织激肽释放酶10CHARACTERISTICSOFTHEGENEEXPRESSIONPROFILEOFOVARIANCLEARCELLCARCINOMAANDVALIDATIONOFSOMERELATEDIMPORTANTGENESABSTRACTPartI:Establishmentoft

3、hegeneexpressionprofileofovarianclearcellcarcinomaObjective:Tostudythegeneexpressionprofileofovarianclearcellcarcinomatoapproachthedifferentialexpressedgenesinovarianclearcellcarcinoma.Method:ThetotalRNAof8clearcellcarcinomaand8serouscystadenocarcinomawer

4、eisolatedusingTRIzolmethod,thenreverselytranscriptedtocDNAwithCy3andCy5labelingrespectively.QuantityoflabeledcDNAwasmixedandhybridizedtothecDNAmicroarray,whichcontainsmorethan14000unigeneandEST.Afterwashing,scanningandimageprocessing,thedifferentialexpres

5、sedgenesbetweentheprofileofclearcellcarcinomaandserouscystadenocarcinomawereanalyzed.Results：82genesthatweredifferentiallyexpressedbetweenovarianclearcellcarcinomaandserousadenocarcinomatissueswereidentified；25geneswerehighlyexpressed>2.0-foldand57geneswe

6、repoorlyexpressed>2.0-foldinovarianclearcellcarcinomatissuerespectively.whichweregroupedintoproliferative,invasion,chemoresistance，cytoskeleton，oncogene，transcriptionregulatorgenesandESTaccordingtofunctions．Conclusion：Establishmentofthegeneexpressionfilec

7、ontributestotheexplorationofthemolecularbiologiccharacteristicofovarianclearcellcarcinoma.KEYWORD：cDNAmicroarray,geneexpressionprofile,ovarianclearcellcarcinomaPartII:SpecialexpressionofannexinIVandkallikrein10inovarianclearcellcarcinomaObjective:Toverify

8、thespecialexpressionofannexinIVandkallikrein10inovarianclearcellcarcinomaaccordingtoitsgeneexpressionprofile.Methods:AnnexinIVandKallikrein10werechosenaccordingtothegeneexpressionprofileofovarianclearcellcarcinomaan