非霍奇金淋巴瘤患者外周血CD4CD25high调节性T细胞研究

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1、非霍奇金淋巴瘤患者外周血CD4+CD25high调节性T细胞研究作者：刘莉，姚军霞，丁乾，黄士昂【摘要】本研究分析B细胞非霍奇金淋巴瘤（B-NHL）患者外周血T细胞亚群及CD4+CD25high调节T(Tr)细胞比例及其变化规律，初步探讨B-NHL免疫抑制机制，以及化疗对此免疫抑制功能的影响。采用流式细胞术检测42例初治B-NHL患者、36例化疗后达CR/PR患者及15例正常健康者（对照组）外周血CD3+、CD4+、CD8+和CD4+CD25highT细胞的比例。结果显示：　B-NHL患者CD3+及CD4+T细胞比例、CD4/CD8比值均低于正常对照组，它们依次为（68.

2、33±15.27）%，对照(72.06±9.26)%；(34.47±12.84)%，对照(42.45±9.2)%；1.36±0.26，对照1.92±0.20，但CD4+CD25highTr细胞比例明显高于正常对照组，为（4.10±1.21）%，对照(2.04±1.03)%，（P<0.001）。化疗后组CD4/CD8比值低于化疗前组（P<0.05）；而外周血CD4+CD25highTr细胞比例，显著高于化疗前组和对照组（P<0.01）。结论：B-NHL初治化疗前及化疗后患者外周血CD4+CD25highTr细胞比例都显著升高，提示CD4+CD25highT

3、r细胞可能是B-NHL患者免疫抑制的重要原因之一。【关键词】非霍奇金淋巴瘤13　　CD4+CD25highRegulatoryTCellsinPeripheralBloodofPatientswithBCellNon-Hodgkin’sLymphoma　　AbstractThisstudywasaimedtoanalyzetheproportionofTcellsubsets，CD4+CD25highregulatongTcells(Tr)inperipheralbloodofB-NHLpatientsandthierchangeregularity，andtoinves

4、tigatetheimmunosuppressionmechanismandinfluenceofchemotherapyonimmunosuppressionfunctionofB-NHLpatients.Theperipheralbloodwascollectedfrom42patientswithB-NHL，36patientswithB-NHLwhoachievedpartialremission(PR)orcompleteremission(CR)after4-6cyclesofchemotherapyand15healthycontrols.Byusingmo

5、noclonalantibodies，thebloodsampleswereevaluatedwiththeflowcytometryforlymphocytesubsetsandTrcells.TheresultsshowedthattheproportionofCD3+andCD4+Tcells，andtheratioofCD4/CD8inpatientswithB-NHLgroupwassignificantlylessthanthoseinthehealthycontrols，i.e.（68.33±15.27）%versus(72.06±9.26)%；(34.47

6、±12.84)%versus(42.45±9.2)%；1.36±0.26versus1.92±0.20，buttheprevalenceoftheCD4+CD25highTrcellswassignificantlyhigherthanthoseinthehealthygroup〖（4.10±1.21）%versus(2.04±1.03)%，P<0.001］.TheratioofCD4/13CD8inchemotherapygroupwaslowerthanthatincontrol，buttheproportionofCD4+CD25highTregcellsin

7、chemotherapygroupwashigherthanthosebeforechemo-/radio-therapyandthecontrol.ItisconcludedthattherelativeincreaseofCD4+CD25highTrcellsinperipheralbloodofB-NHLparientsmayberelatedtoimmunosuppressionandtumorprogression.　　Keywordsnon-Hodgkin′slymphoma；T-cellsubset；chemot