PDL1、PDL2在口腔扁平苔藓中的表达研究

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1、上海交通大学硕士学位论文PD-L1、PD-L2在口腔扁平苔藓中的表达研究姓名：秦孝鹏申请学位级别：硕士专业：口腔临床医学（口腔内科学）指导教师：唐国瑶20080401上海交通大学医学院2005级硕士学位论文正常粘膜上皮层PD-L1的阳性表达率为50%(5/10)，OLP组上皮层PD-L1的阳性表达率为73.3%(22/30)，两组之间无显著性差异(P>0.05)。两组中固有层PD-L1的表达为阴性，两组之间无显著性差异(P>0.05)。两组中上皮层PD-L2的阳性表达率均为100%(10/10，30/30)，两组之间无显著性差异(P>0.05)。正常粘膜固有层PD-L2的阳

2、性表达率为30%(3/10)，OLP组固有层的阳性表达率为100%(30/30)，两组之间有显著性差异(P<0.01)。结论：PD-L1、PD-L2mRNA的表达水平和蛋白表达水平基本一致。PD-L2可能在OLP的发病机制中起重要作用，而PD-L1可能并未参与OLP的发病。关键词：PD-L1,PD-L2,荧光实时定量PCR,免疫组织化学，口腔扁平苔藓II上海交通大学医学院2005级硕士学位论文STUDYONTHEEXPRESSIONOFPD-L1ANDPD-L2INORALLICHENPLANUSABSTRACTObjective:Tostudytheexpressiono

3、fPD-L1andPD-L2inorallichenplanus.Methods:Real-timePCRandimmunohistochemistrytechniqueswereappliedrespectivelytodetectPD-L1、PD-L2mRNAandproteinin30OLPlesionscomparedwithnormalmucosaof10healthyvolunteersascontrols.Results:TheexpressionlevelofPD-L1mRNAinOLPlesionswas1.4timesmorethanthatofnorm

4、almucosa,buttherewasnosignificantdifferencebetweenthem(P>0.05).TheexpressionlevelofPD-L2mRNAinOLPlesionswas3.1timesmorethanthatofnormalmucosa,andtherewassignificantdifferencebetweenthem(P<0.01).TheexpressionlevelofPD-L1mRNAinlesionswithoutepithelialdysplasiaandwithmildepithelialIII上海交通大学医学

5、院2005级硕士学位论文dysplasiawas1.3times、1.7timesmorethanthatofnormalmucosarespectively,buttherewasnosignificantdifferenceamongthem(P>0.05).TheexpressionlevelofPD-L2mRNAinlesionswithoutepithelialdysplasiawas3.0timesmorethanthatofnormalmucosa,andtherewashighsignificantdifferencebetweenthem(P<0.01)；

6、TheexpressionlevelofPD-L2mRNAinlesionswithmildepithelialdysplasiawas3.2timesmorethanthatofnormalmucosa,andtherewashighsignificantdifferencebetweenthem(P<0.01);andtherewasnosignificantdifferencebetweenmildepithelialdysplasiagroupandwithoutepithelialdysplasiagroup(P>0.05).ThepositiverateofPD

7、-L1was50%(5/10)intheepitheliallaminaofnormaloralmucosa,andinOLPwas73.3%(22/30),buttherewasnosignificantdifferencebetweenthem(P>0.05).TheexpressionofPD-L1wasnegativeinlaminapropriaofnormaloralmucosaandOLP,andtherewasnosignificantdifferencebetweenthem(P>0.05).Th