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1、中国农业大学博士学位论文反胶束萃取大豆蛋白质结构与特性研究姓名：赵晓燕申请学位级别：博士专业：食品科学指导教师：陈复生;薛文通20070601中国农业大学博士学位论文AbstractThemVe嫩micelleshavebeenw柚elyappliedinmimicbiologicalfilmcbemis埘and哪rsginflofprotein勰aI跏solvent删加咖ofbiotehclmology．Soybeanhasbeen怕∞印i翻笛ofleoftheimportantproteinsourcesinthefieldoffoodproces
2、singbecauseoftheirnutritionalandfunctionalproperties．Soitisveryinterestingthatthe艇fve嘲micolleswereappliedinsimultaneousextractionofoilandproteinsfromsoybeanflour．Themainconclusiomareasfollowing：1．Theinhibitoryaaivityof件!versemicelles(AOT,SDS，TritonX-100。andcrAB)wasstudied．Theres
3、ultsshowedtheantimicmbiulactivityorderof白∞FcvcrsemicelleswasA饼>SDS>1Ht∞X-100>CTAB．Differentreversemicellashaddifferentantimicrobialactivity,thesurfactantsw啪cKeflyresponsibleforantimicrobialactivityinreVefsemicolles,thehighertheconcentrationof＆Ⅱ丘B切吣was,．thestrongertheantimicrobia
4、lactivityofreversemicelleswas．2．ThedynamicmodelofAOT化Ve髓micellewasstudied．11埒viscosityand$11ffar．4{tensioninit-wellomicell嚣wcroeh2n鲥withdifferentsurfac岫atconcentration,whichwouldc舶mthechange!iofW0aggregates，size,andshapeofmicolles．Undergivensta'f扯tantconcentration,theviscosity,a
5、ggregatesandradiusof"waterpool”inrevclsjemicellesilIaⅨlscdasWowasincreased,andtheshapeofi"cvcirsemicelleswoddbechanged．Inaddition,thediffemntconductivityinreversemicellcscouldreflectthedifferentconformationofreveresmicelle．AtthebeginningtheconductivitywouldbeincreasedastheW0wasi
6、ncreased,thendecreased．3．TheeffectofdifferentsurfactantsandsaltsolutionsinrcveTsemicellesonproteinextractionwasstudied．11kresultsshowedtheanionsurfactentsAOTandSDSinreversemicolleswouldbebenefitforproteinsohibilization,thepercentageofproteinextractionwas68．36and72．9钟乱respectivel
7、y．Insaltsolutions,theKCIandNaN03solutionsinfwersemicelleswouldbenefitforproteinextraction,theassignmentofproteinwas56．71and18．15’‰respectively．4．11硷forwardandbackwardextractionofAOTreversemicolleswasstudiedbyQuadraticGeneralRotaryUnitizedDesignandOrthogonalTestDesign,respectivel
8、y,andfoundtheregressequation．Theresultsshowedth
