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1、采用高效液相色谱-电喷雾串联质谱定量测量西兰花、豆芽、甘蓝芽的硫代葡萄糖苷含量ANALYTICALBIOCHEMISTRYAnalyticalBiochemistry343(2005)93–99www.elsevier.com/locate/yabioQuantitativedeterminationofintactglucosinolatesinbroccoli,broccolisprouts,Brusselssprouts,andcauli?owerbyhigh-performanceliquidchromatography–electrospray
2、ionization–tandemmassspectrometryQingguoTian,RobinA.Rosselot,StevenJ.Schwartz*DepartmentofFoodScienceandTechnology,TheOhioStateUniversity,Columbus,OH43210-1007,USAReceived25February2005Availableonline23May2005Abstract—————————————————————————————————————————————————————Manymethod
3、shavebeenproposedtoanalyzeglucosinolates,aclassofphytochemicalswhosebreakdownproductsarethoughttoberesponsibleforanimprovementinhealth;however,fewarequantitativeandmanyaretimeconsuming.Aselectiveandsen-sitivequantitativemethodfordirectdeterminationofintactglucosinolateswasdevelop
4、edusingliquidchromatographycoupledtoelectrosprayionizationtandemmassspectrometrywithselectedreactionmonitoringdetection.Detectionlimitsforglucoiberin,sin-igrin,progoitrin,glucoerucin,andglucotropaeolinwere1.75,1.38,1.36,0.6,and0.63pmol,respectively.Intraassayprecisionofthemethodw
5、aswithin10%foreachcompound.Themethodwassuccessfullyappliedtoquantify10individualglucosinolatesinbroc-coli,broccolisprouts,Brusselssprouts,andcauli?ower.Theadvantageoftheproposedmethodincludesanalysisofindividualintactglucosinolatesratherthantheconversiontodesulfoglucosinolates,an
6、increasedselectivitythroughtheuseofmassspectrometry,anda10-foldimprovementindetectionsensensitivityoverconventionallyusedHPLCtechniques.ó2005ElsevierInc.Allrightsreserved.Keywords:Glucosinolates;Cruciferousvegetables;Brassicaspp.;LC-ESI/MS/MS——————————————————————————————————————
7、———————————————Glucosinolates(b-thioglucoside-N-hydroxysulfates)areauniquegroupofsulfur-containingplantsecondarymetabolitesfoundincruciferousvegetablessuchasbroccoli,Brusselssprouts,cauli?ower,andkale.AsshowninTable1,allglucosinolatescontainab-D-gluco-pyranosemoietylinkedtoasulfu
8、ratomandeitheranaliphatic,aromatic,orind
