GB 4789.2-2010标准(英文版)

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1、.NationalStandardofthePeople'sRepublicofChinaGB4789.2-2010NationalFoodSafetyStandardFoodMicrobiologicalExamination:AerobicPlateCountIssuedon2010-03-26Implementedon2010-06-01IssuedbyMinistryofHealthofthePeople’sRepublicofChinaGB4789.2-2010ForewordThisStandardreplacesGB/T4789.2-2008Micro

2、biologicalExaminationofFoodHygiene-AerobicPlateCount.ThemainchangesinthisStandardoverGB/T4789.2-2008areasfollows-modifyingbothEnglishandChinesenamesofthisStandard;-modifyingexplanationofthecalculationformulaforaerobicplatecount;-changingtheculturemediumandreagents;and-deletingthesecond

3、methodofaerobicplatecount,PetrifilmTMteststripmethod.AnnexAofthisStandardisnormative.ThepreviouseditionsreplacedbythisStandardareasfollows:-GB4789.2-1984,GB4789.2-1994,GB/T4789.2-2003andGB/T4789.2-2008.IGB4789.2-2010NationalFoodSafetyStandardMicrobiologicalExamination:AerobicPlateCount

4、1ScopeThisStandardspecifiesadeterminationmethodofaerobicplatecount.ThisStandardisapplicabletothedeterminationoftheaerobicplatecount.2Termsanddefinitions2.1AerobicplatecountItreferstothetotalmicrobescoloniesamountinperg(mL)oftestingfoodsampleafterthesamplehasbeentreatedandculturedunderc

5、ertainconditions(e.g.culturemedium,culturetemperatureandincubationtime).3ApparatusandmaterialsBesidestheconventionalapparatusforsterilizationandcultureinamicrobiologicallab,otherrequiredequipmentandmaterialsareasfollows:3.1Constant-temperatureincubator:36℃±1℃,30℃±1℃3.2Refrigerator:2℃–5

6、℃3.3Constant-temperaturewaterbath:46℃±1℃3.4Balance:accurateto0,1g3.5Homogenizer3.6Oscillator3.7Sterilepipettes:1mL(accurateto0,01mL),10mL(accurateto0,1mL)ormicropipetteswithsuctionhead3.8Sterileconicalflasks:250mLand500mL3.9Sterilepetridish:90mmindiameter;3.10pHmeterorpHcolorcomparison

7、tubeorprecisionpHpaper3.11Magnifierand/orcolonycounter4Culturemediaandreagents4.1Platecountagarmedium:SeeA.1inAnnexA4.2Phosphatebuffersolution:SeeA.2inAnnexA.4.3Sterilenormalsaline:SeeA.3inAnnexA.5ExaminationprocedureFig.1showsthedeterminationprocedureoftheaerobicplatecount.5GB4789.2