nedd9在肝癌中的表达及其与肝癌细胞生物学特性之间关系的研究

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1、分类号R735.7UDC616-006密级公开NEDD9在肝癌中的表达及其与肝癌细胞生物学特性之间关系的研究卢鹏培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)外科学（普外）研究方向肝癌的基础与临床指导教师窦科峰教授（主任医师）培养单位西京医院肝胆胰脾外科二O一五年五月第四军医大学博士学位论文目录缩略语表·······························································································1中文摘要·················

2、··············································································3英文摘要·······························································································7前言······························································································11文献回顾·

3、·····························································································13正文······························································································26第一部分NEDD9在人肝细胞癌组织中的表达及意义································261材料（包括所涉及的手术切除标本、所用的试剂耗材以及设备仪器）

4、·······261.1试验中所涉及的组织标本···························································261.2实验中所用的试剂及耗材···························································261.3实验中所用到的设备和仪器························································272方法···············································

5、·············································282.1组织标本的采集以及临床病理资料的获取······································282.2RT-qPCR方法分析组织细胞中NEDD9mRNA的表达水平·················282.3Westernblot检测组织中NEDD9蛋白的表达···································302.4免疫组织化学染色·············································

6、·······················332.5读片·······················································································342.6随访和预后评估·······································································352.7统计分析·········································································

7、·······353结果····························································································353.1不同组织中NEDD9mRNA表达水平的差异···································353.2不同组织中NEDD9蛋白表达水平的差异·······································363.3NEDD9免疫组织化学染色····································

8、······················363.