跟腱干细胞及骨髓间充质干细胞移植在大鼠跟腱再生中的研究

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1、䐕㞧ᒨ㓼㜔਀僞儉䰪䍞ᒨ㓼㜔〱ὃ൞ཝ啖䐕㞧߃⭕ѣⲺ⹊ガ䠃ᒼཝᆜঐ༡ᆜփ䇰ᮽᆜ⭕ဉ੃φALANIMOHANADKHALIDAHMED(ᶄࢇ)᤽ሲᮏᐾφᶞ࣑ᮏᦾщѐφ⭕⢟ॱᆜᐛぁᆜ〇䰞㊱φᐛᆜ䠃ᒼཝᆜ⭕⢟ᐛぁᆜ䲘ӂOжӊᒪॷᴾStudyofTendon-DerivedStemCellandBoneMarrow-derivedMesenchymalTransplantationontheRegenerationEffectoftheAchillestendonRuptureinRatsAThesisSubmittedtoChongqingUniversity

2、InPartialFulfillmentoftheRequirementsfortheDoctor’sDegreeofEngineeringByAlaniMohanadKhalidAhmedSupervisedbyProf.LiYangSpecialty:BiomedicalEngineeringChongqingUniversity,CollegeofBiongineeringChongqing,ChinaOctober2015ѝ᮷᪈㾱᪎㾷ⴞⲴ˖㚼㞡ᱟа㊫⭡ᒣ㹼ᧂࡇⲴ㓔㔤㜦৏ᶏᶴᡀⲴ㠤ᇶ㔃㕄㓴㓷ˈ䘎᧕㚼㚹-僘ˈ᭟ᤱ䓛փⲴ䘀ࣘDŽ䲿⵰ӪԜ⭏⍫≤ᒣⲴᨀ儈

3、ˈ䘀ࣘᡀѪа⿽⍱㹼ⲴǃڕᓧⲴ⭏⍫ᯩᔿˈ㚼㞡ᦏՔⲴ⯵ҏ䗵䙏໎ཊˈަѝˈᴹ35%኎Ҿ䐏㞡ᯝ㻲DŽ㚼㞡ᦏՔਾˈ⭡Ҿ㓴㓷޵㹰׋䖳ቁˈ㓴ᡀⲴᡀ㓔㔤㓶㜎ԓ䉒䖳վˈ؞༽㕃ធˈфᚒ༽ࡠᦏՔࡽ≤ᒣ䖳䳮DŽⴞࡽˈᑨ㿱Ⲵ㚼㞡ᦏՔ⋫⯇ᯩ⌅ਟ࠶Ѫ˖؍ᆸ⋫⯇઼᡻ᵟ⋫⯇DŽ؍ᆸ⋫⯇ӵӵ㜭㕃䀓⯷⣦ˈф㙇ᰦ䮯ˈ⯵Ӫ䴰㾱㓿শ䮯ᵏⲴᓧ༽䗷〻ˈѤᒺ⯇᭸н㜭ԔӪ┑᜿˗᡻ᵟ⋫⯇䲔Ҷᑨ㿴Ⲵ᡻ᵟ㕍ਸཆ,䘈׍䎆Ҿ〫Ἵ⢙ˈवᤜ㠚փǃᔲփǃٷփ〫ἽDŽնᱟ䘉Ӌ〫Ἵ⋫⯇ᯩ⌅৸ᴹަ⢩ᴹ㕪䲧ˈྲ㠚փ〫Ἵ䴰㾱⢪⢢㠚䓛׋४Ⲵ㓴㓷ˈᱟа⿽¯᣶ь້㺕㾯້āⲴᯩ⌅ˈ䘈ਟ㜭Պᕅਁ׋४ࠪ⧠ᒦਁ⯷ˈՊሬ㠤⯵Ӫ仍ཆⲴࡋՔ˗ᔲփ〫Ἵࡉᆈ

4、൘㓴㓷ᶕⓀഠ䳮ǃᔲփ⯮⯵Րḃ仾䲙ǃٷ˗ㄟᔺㅹᯕᧂ⯛փ〫Ἵᆈ൘〫Ἵ⢙ᮤਸᐞǃ䘌ᵏ⯇᭸нㅹ㕪⛩DŽഐ↔ˈᴹᗵ㾱᧒㍒ᯠⲴǃᴤ࣐ᴹ᭸Ⲵ䐏㞡ᦏՔ⋫⯇䙄ᖴDŽᒢ㓶㜎ާᴹ䲶ᖒᡀǃཊੁ࠶ॆǃ㠚ᡁᴤᯠⲴ⢩ᙗˈᱟ㓴㓷޽⭏Ⲵᗵ㾱ഐ㍐DŽབྷ䟿⹄ウ㺘᰾ˈ僘儃䰤䍘ᒢ㓶㜎˄bonemarrow-derivedmesenchymalstemcells,BMSCs˅ާᴹ▌൘࠶ॆѪཊ⿽㔃㕄㓴㓷Ⲵ⢩ᙗˈवᤜ˖僘ǃ䖟僘ǃ㚼㞡ǃ㚼㚹ǃ僘儃ǃ㜲㛚ㅹDŽᴹᣕ䚃㺘᰾ˈ僘儃䰤䍘ᒢ㓶㜎㜭׳䘋㚼㞡㓴㓷Ⲵ޽⭏؞༽DŽ⭡㚼㞡ǃ丗ᑖ㓴㓷࠶⿫Ⲵ㚼㞡ᒢ㓶㜎˄tendon-derivedstemcells,TDSCs)ᱟа⿽

5、ᡀփᒢ㓶㜎ˈਟ৲о㚼㞡ᦏՔⲴ؞༽䗷〻DŽᴹᣕ䚃㺘᰾ˈTDSCs䖳BMSCsާᴹᴤᕪⲴ䲶ᖒᡀ㜭࣋ˈᴤᘛⲴ໎⇆䙏⦷DŽ൘㳻ⲭ㺘䗮䉡৺สഐ㺘䗮≤ᒣкˈTDSCsᱟ䖳BMSCsᴤ৏࿻Ⲵа⿽ᒢ㓶㜎⿽㊫DŽ਼ᰦˈTDSCs儈㺘䗮㚼㞡㓴㓷⴨ޣḷᘇ⢙˖∄ྲ䖜ᖅഐᆀscleraxis(Scx)઼tenomodulin(Tnmd)ˈԕ৺㓶㜎ཆส䍘ᡀ࠶CollagenIǃCollagenIII઼decorin(Dcn)ㅹDŽഐ↔TDSCsਟ㜭ᱟа⿽ᴤ࣐Ո㢟Ⲵਟ⭘Ҿ׳䘋㚼㞡㓴㓷޽⭏؞༽Ⲵ⿽ᆀ㓶㜎DŽնᱟˈሩҾTDSCs৺BMSCs⭘Ҿ㚼㞡ᯝ㻲؞༽Ⲵ֌⭘ᐞᔲᵚ㿱㌫㔏Ⲵ⹄ウᣕ䚃DŽഐ↔ˈᵜ

6、᮷䟷⭘SDབྷ啐Ѫ⹄ウሩ䊑ˈᨀਆ࠶⿫є⿽ᒢ㓶㜎DŽ൘փཆ㘳ሏє㘵Ⲵสഐ䖜ᖅǃ㳻ⲭ㺘䗮ǃ㓶㜎㺘රǃ໎⇆䙏⦷ㅹᐞᔲ˗൘↔⹄ウส⹰кˈԕབྷ啐䐏㞡ᯝ㻲Ѫ⯮⯵⁑රˈ㘳ሏ㓶㜎〫ἽሩҾ㚼㞡ᯝ㻲؞༽Ⲵ֌⭘DŽᯩ⌅˖ᵜ⹄ウѝˈᡁԜ俆ᡀ࣏ⲴӾབྷ啐僘儃о䐏㞡ѝ࠶⿫ᗇࡠҶBMSCs઼TDSCsˈ䙊䗷⍱ᔿ㓶㜎Ự⍻ǃཊੁ࠶ॆ䈡ሬᇎ傼⍻ᇊަᒢ㓶㜎኎ᙗDŽ↔ཆˈ䘎㔝㿲ሏє⿽ᒢ㓶㜎⭡ㅜаԓ㠣ㅜгԓⲴ㓶㜎ᖒᘱˈ㘳ሏє⿽ᒢ㓶㜎Ⲵ㓶㜎ᖒᘱᐞᔲ৺⭏䮯⣦ᘱDŽIChongqingUniversity,PhDThesis൘փཆᇎ傼ѝˈᡁԜ䙊䗷RT-PCRǃ⯛㦗ḃ㢢ᇎ傼ǃEdU㓶㜎໎⇆ᇎ傼ǃMTSᇶᓖ∄㢢㓶

7、㜎⭏ᆈ⍫࣋Ự⍻ᇎ傼࠶࡛∄䖳ҶTDSCs઼BMSCsⲴOct-4ˈNucleosteminˈP75ˈNanogˈα-SMAˈBiglycanˈVimentinˈDecorinˈScleraxisˈTenascin-CˈCollagenIIIˈCollagenIIˈCollagenIˈSDF-1สഐ㺘䗮ᐞᔲǃCD29,CD44,CD90,CD146,Oct-4,α-SMA৺Nucleostemin㳻ⲭ㺘䗮䉡ᐞᔲǃDNAਸᡀ䙏⦷ԕ৺㓶㜎໎⇆ⲴᐞᔲDŽ൘փ޵ᇎ傼ѝˈᡁԜ俆ᔪ・ҶSDབྷ啐䐏㞡ᯝ㻲⁑රˈ൘䐏㞡ᦏՔਾሶє⿽ᒢ㓶㜎࠶࡛〫Ἵࡠབྷ啐䐏㞡ᯝ㻲༴ˈਆн਼ᰦ䰤⛩

8、˄1ઘǃ2ઘǃ3ઘ˅ˈӾཆ㿲Ựḕǃᗞ㿲㓴㓷ᆖ䇴ԧǃ⭏⢙࣋ᆖ⍻ᇊᶕ㘳ሏє⿽ᒢ㓶㜎ሩҾᯝ㻲㚼㞡Ⲵ؞༽֌⭘DŽ਼ᰦˈ