唐古特白刺果多糖与黑果枸杞多糖对免疫受抑小鼠ifn-γ及tnk细胞的影响

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1、分类号：R392密级：公开UDC：610编号：201506121001021082012级攻读硕士学位研究生毕业论文唐古特白刺果多糖与黑果枸杞多糖对免疫受抑小鼠IFN-γ及T/NK细胞的影响EffectsofPolysaccharidesofNitrariatangutorumBobrandLyciumruthenicumMurr.onIFN-γandT/NKCellsinImmunosuppressedMice指导教师：耿排力教授学生姓名：秀仁杰学科专业名称：基础医学（免疫学）研究方向：药理免疫学院(系、部)：青海大学医学院二零一五

2、年六月QinghaiUniversityMasterDissertationEffectsofPolysaccharidesofNitrariatangutorumBobrandLyciumruthenicumMurr.onIFN-γandT/NKCellsinImmunosuppressedMiceSupervisor:PailiGengCandidate:RenjieXiuAcademicMajorApplidedfor:ImmunologySpecificMajor:ImmunopharmacologyCollege(Depar

3、tment):MedicalCollegeofQinghaiUniversityJune,2015目录中文摘要.........................................................IIIAbstract...........................................................V引言...............................................................1第一章超声-微波协同提取唐古特白刺果多糖与

4、黑果枸杞多糖............31.1材料与方法.................................................31.2实验方法..................................................41.3结果NTB多糖与LRM多糖得率及含量.........................61.4小结......................................................7第二章NTB多糖与LRM多糖对免疫受抑小鼠IFN-、T

5、/NK细胞的影响.......92.1实验材料...................................................92.2实验动物及分组............................................102.3小鼠脾脏、胸腺重量及组织结构观察察........................102.4小鼠脾细胞IFN-提取......................................112.5小鼠血清IFN-的测定.......................

6、...............122.6T/NK细胞亚群分析.........................................122.7数据处理..................................................122.8结果......................................................12第三章讨论......................................................183.1免疫抑制低下动物模型的建立

7、.................................223.2NTB多糖与LRM多糖对免疫受抑小鼠免疫调节作用...............233.3结论.......................................................24参考文献..........................................................26综述..............................................................3

8、0唐古特白刺果及黑果枸杞生物活性研究进展.........................30一唐古特白刺果研究现状........................................30二黑果枸杞研究现状.