枸杞多糖对血虚模型小鼠血细胞的影响.pdf

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1、·3662·现代中西医结合杂志ModernJournalofIntegratedTraditionalChineseandWesternMedicine2014Nov，23(33)枸杞多糖对血虚模型小鼠血细胞的影响杨金枝，崔晓鸽(郑州澍青医学高等专科学校，河南郑州450064)[摘要]目的观察枸杞多糖对放血和环磷酰胺致血虚模型小鼠血细胞的影响。方法采用腹腔注射环磷酰胺和放血方法复制小鼠血虚模型。将实验小鼠分为6组，每组15只。空白组于第2，4，6，8天腹腔注射生理盐水0．1mL／10g；其余各组于

2、第2，4，6，8天腹腔注射环磷酰胺40mg／kg，第9天每鼠尾部放血0．6mL／20g，制作血虚模型；枸杞多糖大、中、小剂量组第1天按10mL／(kg·d)灌胃枸杞多糖，剂量分别为26．4，l3．2和6．6g／kg，阿胶组第l天按10mL／(kg·d)灌胃阿胶3g／kg。实验过程中，每天称1次小鼠的体质量，观察，J、鼠的精神活动。用药第10天灌胃2h后，处死小鼠，摘取眼球取血液，检测红细胞(RBC)、白细胞(WBC)、血红蛋白(Hh)及血小板(Ph)计数。结果枸杞多糖大、中剂量组的WBC、RBC、

3、Hb和Plt值均明显高于模型组(P均<0．01)。结论枸杞多糖能够升高血虚模型小鼠的WBC、RBC、Hb和PIl的含量。[关键词]枸杞多糖；血虚模型；放血；环磷酰胺doi：10．3969／j．issn．1008—8849．2014．33．005[中图分类号]R一332[文献标识码]A[文章编号]1008—8849(2014)33—3662—03InfluenceofWolfberryp0lysaccharideonthebloodcelloftheblooddeficiencymiceYangJi

4、nzhi，CuiXiaoge(ShuqingMedicineCollegeofZhenzhou，Zhengzhou450064，Henan，China)Abstract：0bjectiveItistoobservetheinfluenceofWolfberrypolysaccharideonthebloodcelloftheblooddeficiencymicecausedbyintraperitonealinjectionofNeosar．MethodsThemodelofblooddefici

5、encymicebybloodlettingandintraper—itonealinjectionofNeosar．Allthemouseweredividedinto6groups，15casesineachgroups．Animalsinblankcontrolgroupweretreatedwithphysiologicalsaline0．1mL／lOgbyintraperitonealinjectiononthe2rd，4th，6thand8thday；alltheothersweret

6、reatedwithcyclophosphanebyintraperitonealinjectiontoonthe2rd，4th，6thand8thday，andbloodlettingfor0．6mL／20gformtailonthe9thdayforeachmousetoestablisheddeficiencymode1．Wolfberrypolysaccharidehighdose，mediumdose，smalldosegroupweretreatedwithWolfberrypolys

7、accharidebyorallyonthefirstdayfordoseof26．4，13．2，6．6g／kgrespectively，andwithdonkey—hidegelatinfordoseof3g／kgatthesametime．Duringtheexperiment，theweighingwereweighedandthepsychomotilitywereobservedeveryday．OnthelOthdayafterorallyfor2一hour，theanimalswer

8、ekilledandabstractedtheeyeballbloodtomeasureRBC，WBC，HbandPit．ResultsThevaluesofWBC，RBC，HbandPltinWolfberrypolysaccharidehighdoseandmediumdosegroupweremarkedlyincreasedthanthatofmodelgroup(allP<0．01)．Conclu-sionWolfberrypolysaccharidecanincreas