sirna沉默xiap基因对骨肉瘤mg-63细胞生物学特性的影响

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1、授予单位代码10089学号或申请号13321HebeiMedicalUniversity硕士学位论文在职科学学位SiRNA沉默XIAP基因对骨肉瘤MG-63细胞生物学特性的影响学位申请人：王井伟导师：尹晓琳教授专业：免疫学_>级学院：基础医学院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独立完成。本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公幵和使用。凡发表与学位论文主要内容相关的论文，第一署名为单位河北医科大学，试验材料、原始数据、申报

2、的专利等知识产权均归河北医科大学所有。否则，承担相应法律责任。研究生签名导师签章:级学院领导盖章年/月巧曰河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人己经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写，文责自负。研究生签名：导•签章年义月j日目录中文摘要··············································································1英文摘要············

3、··································································3英文缩写··············································································5研究论文SiRNA沉默XIAP基因对骨肉瘤MG-63细胞生物学特性的影响前言··············································································6材料与方法········

4、·····························································7结果·············································································11附图·············································································14附表······················································

5、·······················19讨论·············································································20结论·············································································23参考文献·······································································23综述骨肉瘤治疗最新研究进展···

6、··············································27致谢···················································································38个人简历·············································································39中文摘要SiRNA沉默XIAP基因对骨肉瘤MG-63细胞生物学特性的影响摘要目的：骨肉瘤是一种临床常见的好发于青少年的成骨性

7、恶性肿瘤，它恶性程度高，预后比较差。随着化学疗法、手术技术、骨重建等方法的进展，5年总生存率明显提高。阿霉素(adriamycin,ADM)、顺铂（diamminedichloroplantinum,DDP）是抗骨肉瘤的最有效药物,但肿瘤细胞对化疗药物产生耐药性是影响化疗疗效的主要原因。许多化疗药物通过诱导凋亡抗肿瘤生长。XIAP是凋亡调节因子家族中对凋亡调节作用最强的分子之一，同时是唯一的caspases抑制剂。研究显示它在多种恶性肿瘤中上调表达，并与肿瘤化疗耐药及预后差紧密相关。RNAi是近年来发展起来的新技术,siRNA已经成为继反义核酸、核酶之后

8、基因治疗的又一新武器。为此,我们合成特异性干扰XIAP基因的siRNA，并在脂质