雌激素对髓鞘再生的影响及机制研究

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1、授予单位代码10089学号或申请号13007对妗(E科大交HebeiMedicalUniversity硕士学位论文在职科学学位雌激素对髓鞘再生的影响及机制研究学位申请人：田亚汀导师：马春玲教授张国徽教授专业：法医学二级学院：基础医学院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师(或指导小组)的指导下，由本人独立完成�本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公幵和使用》凡发表与学位论文主要内容相关的论文，第一署名为单位河北医科大学，试验材料、原始

2、数据、申报的专利等知识产权均归河北医科大学所有。否则,承担相应法律责任。研究生签名：导师签章：二级学院领导盖章：>5年月勾曰河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果,指导教师对此进行了审定。本论文由本人独立撰写,文责自负。研究生签名：⑩g导师签章：>A年&月工I曰目录中文摘要······································································

3、·······1英文摘要·············································································4英文缩写·············································································7研究论文雌激素对髓鞘再生的影响及机制研究前言············································································

4、·8材料与方法····································································9结果·············································································17附图·············································································21附表······························

5、···············································34讨论·············································································37结论·············································································40参考文献·······················································

6、················40综述雌激素对中枢神经系统损伤保护的研究现状·······················44致谢···················································································58个人简历·············································································59中文摘要雌激素对髓鞘再生的影响及机制研究摘要目的：评价雌激素对髓鞘再生的

7、影响并探讨其可能的机制，为脱髓鞘疾病损伤的治疗提供新的思路。方法：66只雌性6周龄C57BL/6小鼠适应性喂养一周后，随机分为6组（n=11）：正常组、脱髓鞘模型组、自然恢复组（脱髓鞘后自然恢复2周）、雌激素治疗组（脱髓鞘后雌二醇E2（5mg/kg体重，治疗2周）、卵巢去势组（脱髓鞘后卵巢去势，2周后观察）和假手术组（脱髓鞘后假手术，2周后观察）。小鼠脱髓鞘模型以0.2%Cuprizone（CPZ）混合饲料连续饲喂6周方式诱导。通过免疫组织化学染色方法检测小鼠脑组织内髓鞘碱性蛋白MBP（Myelinbasicprotein）的表达，评

8、价髓鞘脱失及再生的程度；以行为学实验方法检测激素治疗对小鼠运动能力的影响；以westernblot技术及免疫组化实验方法检测小鼠脑组织内MBP、少突胶质细胞前期细胞标记物NG2（nerveglialantigen2）、少