辅酶q10对糖尿病大鼠牙周炎牙龈组织mmp-9和血清opg、rankl的影响

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1、授予单位代码10089学号或申请号13809HebeiMedicalUniversityt学位论文在职科学学位辅酶Qio对糖尿病大鼠牙周炎牙龈组织MMP-9和血清OPG�RANKL的影响学位申请人：张翠娟导师：薛毅教授吴仲寅副教授专业：口腔临床医学二级学院：白求恩国际和平医院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师(或指导小组)的指导下,由本人独立完成。本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用•凡发表与学位论文主要内容相关的论文，

2、第一署名为单位河北医科大学，试验材料、原始数据、申报的专利等知识产权均归河北医科大€有。否则，承担相应法律责任。研究生签名：导师签章:二级学院领导盖詩年r河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写,文责自负。研究生签名：导师签章:�丨1年/月目录中文摘要···························································

3、···················1英文摘要··············································································5研究论文辅酶Q10对糖尿病大鼠牙周炎牙龈组织MMP-9和血清OPG、RANKL的影响前言·············································································10材料与方法·······································

4、·····························11结果·············································································16附图·············································································19附表···········································································

5、··29讨论·············································································33结论·············································································40参考文献·······································································40综述辅酶Q10对糖尿病大鼠牙周炎牙龈组织MMP-9和血

6、清OPG、RANKL的影响····················································45致谢····················································································53个人简历··············································································54中文摘要辅酶Q10对糖尿病大鼠牙周炎牙龈组织MMP-9和血清O

7、PG、RANKL的影响摘要目的：建立Ⅱ型糖尿病大鼠模型，在此基础上用辅酶Q10进行干预，评价Ⅱ型糖尿病大鼠的牙周病损状态的差异和变化，通过免疫组织化学技术检测大鼠牙周组织中基质金属蛋白酶-9（matrixmetalloproteinases,MMP-9)的表达和酶联免疫吸附实验(ELISA)检测大鼠血清中骨保护素（osteoprotegerin，OPG）、核因子－κＢ受体活化因子配体（receptoractivatorfornuclearfactor-κBligand,RANKL）的水平的改变，探讨辅酶Q10在阻断或延缓糖尿病大鼠牙周

8、炎发病进程中的意义，为辅酶Q10临床辅助治疗或预防糖尿病牙周炎提供理论参考。方法：选取120只健康雄性Wistar大鼠，体重约180g左右，随机分6组，20只为一组，分别为空白组（O组）、牙周炎组（P组）、糖尿病组（DM