尿道下裂患者包皮组织中雄激素受体基因启动子区甲基化状态研究

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1、授予单位代码10089学号或申请号20122497硕士学位论文科学学位尿道下裂患者包皮组织中雄激素受体基因启动子区甲基化状态研究作者姓名：钱光耀导师：廖贤平教授专业：外科学二级学院：武警后勤学院2015年3月可北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独立完成。本学位论文研宄所获的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公共和使用。凡发表与学位论文主要内容相关的论文，第一署名单位为河北医科大学，试验材料、原始数据、申报的专利等知识产权归河北医科大学所有。否则，承担相应的法律责任。IA研宄生签名

2、导师签章:i及学脘签章：.4气牟、月）巧3河北医科大学研究生论文独创性声明本论文是在导师指导下进行的研究工作及取得的研宄成果，除了文中特别加以标注等内容之外，文中不包括其他人己经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写，文责自负。研究生签名:导师签章:年目录中文摘要···········································································1英文摘要··································································

3、·········4英文缩写···········································································7研究论文尿道下裂患者包皮组织中雄激素受体基因启动子区甲基化状态研究前言·················································································8材料与方法······································································10结果······

4、·········································································21附图···············································································23附表···············································································25讨论·················································

5、······························26结论···············································································28参考文献·········································································29综述尿道下裂病因学研究进展·················································32致谢································

6、·····················································45个人简历···············································································46中文摘要尿道下裂患者包皮组织中雄激素受体基因启动子区甲基化状态研究摘要目的：尿道下裂是由于阴茎尿道发育异常而导致的，是男性新生儿中最常见的生殖器发育畸形。尽管研究不断的深入，尿道下裂的病因仍就有许多的未知。然而，目前正处于尿道下裂病因学、流行病学和实验室研究的新的分水岭。据推测，由内源性或外源性因素导致

7、，在阴茎和尿道发育关键期（8至14周）性激素浓度的变化，可能在尿道下裂的发生起一部分作用。因为某些内源性或外源性因素，如激素水平，饮食和接触化学物质可能会导致表观遗传改变，特别是在胎儿发育过程中，母亲所暴露的条件。本实验的研究目的在于探讨雄激素受体基因甲基化状态与表达情况以及双氢睾酮水平是否与尿道下裂的发生有关。方法：将15例尿道下裂患者作为实验组，15例年龄相似的包皮环切术患者作为对照组进行研究。1包皮组织以4%多聚甲醛溶液固定，常规制备病理切片，苏木精-伊红(HE)染色后观察组织病理变化