脑室内注射地塞米松对雌性小鼠能量代谢和生殖功能的影响

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1、分类号：R589.2学校代码：10392学科专业代码：100201学号：2151003473硕士学位论文脑室内注射地塞米松对雌性小鼠能量代谢和生殖功能的影响EffectofIntraventricularInjectionofDexamethasoneonEnergyMetabolismandReproductiveFunctioninFemaleMice学位类型：医学硕士所在学院：省立临床医学院申请人姓名：苏艳玲学科、专业：内科学（内分泌与代谢）导师：陈刚教授研究起止日期：2016年1月至2018年3月答辩委员会主席：杨立勇教授答辩日期：2018年5月11日二○一八年五

2、月福建医科大学硕士学位论文目录缩略词表„„„„„„„„„„„„„„„„„„„„„„„„„1中文摘要„„„„„„„„„„„„„„„„„„„„„„„„„2英文摘要„„„„„„„„„„„„„„„„„„„„„„„„„4前言„„„„„„„„„„„„„„„„„„„„„„„„„„„6第一部分脑室内注射地塞米松对雌性小鼠能量代谢的影响„„„10引言„„„„„„„„„„„„„„„„„„„„„„„„„10材料与方法„„„„„„„„„„„„„„„„„„„„„„11结果„„„„„„„„„„„„„„„„„„„„„„„„„24讨论„„„„„„„„„„„„„„„„„„„„„„„„„30第二部分脑室内注

3、射地塞米松对雌性小鼠生殖功能的影响„„„33引言„„„„„„„„„„„„„„„„„„„„„„„„„33材料与方法„„„„„„„„„„„„„„„„„„„„„„34结果„„„„„„„„„„„„„„„„„„„„„„„„„39讨论„„„„„„„„„„„„„„„„„„„„„„„„„44研究结论„„„„„„„„„„„„„„„„„„„„„„„„47参考文献„„„„„„„„„„„„„„„„„„„„„„„„48综述„„„„„„„„„„„„„„„„„„„„„„„„„„53致谢„„„„„„„„„„„„„„„„„„„„„„„„„„64福建医科大学硕士学位论文缩略词中英文对照表缩略词英文全称中文全

4、称ACTHadrenocorticotrophichormone促肾上腺皮质激素AgRPagouti-relatedprotein刺鼠相关蛋白AnteroventralperiventricularAVPV前腹侧室旁核nucleusARCArcuatenucleus弓状核BATBrwonadiposetissue棕色脂肪组织cocainandamphetamine-regulatedCART可卡因-苯丙胺调节转录物transcriptCRHcorticotropinreleasinghormone促肾上腺皮质激素释放激素DEXdexamethasone地塞米松E2estr

5、adiol雌二醇GCglucocorticoid糖皮质激素GnRHGonadotropin-releasinghormone促性腺激素释放激素GPR54G-protein-coupledreceptor54G蛋白偶联受体GRglucocorticoidreceptor糖皮质激素受体HHhypogonadotropichypogonadism低促性腺激素性性腺功能减退症HPAhypothalamic-pituitary-adrenalaxis下丘脑-垂体-肾上腺轴HPGhypothalamic-pituitary-gonadalaxis下丘脑-垂体-性腺轴LHLuteini

6、zinghormone黄体生成素NPYneuropeptideY神经肽YPOMCpro-opiomelanocortin阿片-促黑素细胞皮质素原PVNparaventricularnucleus室旁核WATWhiteadiposetissue白色脂肪组织福建医科大学硕士学位论文脑室内注射地塞米松对雌性小鼠能量代谢和生殖功能的影响摘要目的：下丘脑-垂体-肾上腺轴参与能量平衡的调节，且对生殖和发育有着复杂的影响，GC（glucocorticoid，糖皮质激素）在外周的作用报道较多，但对其在中枢的直接作用报道较少。因此，本研究通过侧脑室注射地塞米松，探讨其对能量代谢和生殖系统的

7、影响。方法：将24只小鼠随机分成2组，每组12只，通过脑立体定位仪，在小鼠皮下埋置ALZET1004，通过与kit3连接，分别将乙醇（对照组、CON组）和地塞米松（DEX组）缓慢、持续的送入小鼠侧脑室，连续给药28天。1、置管前的当天和置管后每天早上同一时间测量小鼠的体重和摄食量；置管后第27天进行小鼠腹腔葡萄糖耐量试验；置管后第29天处死小鼠，迅速分离小鼠棕色脂肪组织、性腺周围WAT（whiteadiposetissue，白色脂肪组织）、肾脏周围WAT，皮下WAT，并称重；迅速分离下丘脑，实时荧光定量PCR（realtime