固本化瘀通络合剂含药血清对高糖环境下足细胞损伤、凋亡及自噬的影响及分子机制研究

《固本化瘀通络合剂含药血清对高糖环境下足细胞损伤、凋亡及自噬的影响及分子机制研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、成都中医药大学博士学位论文成都中医药大学博士学位论文学位论文固本化瘀通络合剂含药血清对高糖环境下足细胞损伤、凋亡及自噬的影响及分子机制研究TheEffectsandMechanismofMedicin-ContainingSerumofChineseHerbalMedicinalCompoundGubenHuayuTongluoonPodocytesInjury,ApoptosisandAutophagyInducedbyHighGlucose张翠薇指导教师姓名：马跃荣教授申请学位级别：博士专业名称：中西医结合基础论文提交时间：2018.05.2

2、3论文答辩时间：2018.05.30二○一八年五月成都中医药大学博士学位论文目录中文摘要·······························································································1Abstract·································································································4英汉缩略词对照表······················

3、······················································8引言·····································································································10第一部分固本化瘀通络合剂含药血清对高糖环境下足细胞的作用浓度摸索····················································································

4、·······131.材料与方法····························································································131.1实验材料····························································································131.1.1实验动物························································

5、·····························131.1.2实验细胞·····················································································131.1.3实验药材·····················································································141.2主要试剂··············································

6、·············································141.3主要仪器和设备·················································································151.4实验方法···························································································151.4.1固本化瘀通络合剂含药血清及正常大鼠血清的制备·········

7、···········151.4.2细胞培养与分组·········································································161.4.3形态学观察················································································181.4.4CFSE/PI染色······································································

8、······181.5统计学分析····················································