n-乙酰半胱氨酸对改善重症急性胰腺炎胰腺微循环的研究

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1、授予单位代码10089学号或申请号12247HebeiMedicalUniversity硕士学位论文科学学位N-乙酰半胱氨酸对改善重症急性胰腺炎胰腺微循环的研究作者姓名：张瑞坚导师：彭彦辉教授脱红芳副教授专业：外科学二级学院：河北省人民医院2015年03月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师(或指导小组)的指导下，由本人独立完成。本学位论文研究所获得的研究成果,其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文主要内容相关的论文,第一署名为单

2、位河北医科大学，试验材料、原始数据、申报的专利等知识产权均归河北医科大学所有。否则，承担相应法律责任。W—研究生签名:€导师签日一河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外,文中不包含其他人已经发表或撰写的研究成果,指导教师对此进行了审定。本论文由本人独立撰写,文责自负。b：月1^0目录中文摘要·······································································

3、······1英文摘要·············································································3研究论文N-乙酰半胱氨酸对改善重症急性胰腺炎胰腺微循环的研究前言·············································································5材料与方法·······················································

4、·············6结果·············································································11附图·············································································13附表·············································································18讨论

5、·············································································20结论·············································································22参考文献·······································································23综述N-乙酰半胱氨酸在急性胰腺炎中的作用··

6、···························26致谢···················································································33个人简历·············································································34中文摘要N-乙酰半胱氨酸对改善重症急性胰腺炎胰腺微循环的研究摘要目的：本实验首先通过3.5%的牛磺胆酸钠以0.1mg/1

7、00g的剂量经胰管用微量泵逆行注射，诱导大鼠形成重症急性胰腺炎(severeacutepancreatitis,SAP)模型，通过测定大鼠血清淀粉酶（serumamylase，AMS）、血清白介素-6（Interleukin-6，IL-6）水平，检测胰腺组织血栓素B2(ThromboxaneB2,TXB2)、6-酮-前列腺素-F1a(6-ketoprostaglandin-F1,6-Keto-PGF1α)含量，以及对大鼠胰腺组织进行病理学观察，探讨N-乙酰半胱氨酸(N-acetylcysteine,NAC)对大鼠

8、重症急性胰腺炎微循环方面的改善作用。方法：实验选取健康的SD大鼠54只，雌雄各占一半，体重200～250g(河北医科大学动物中心供给)，随机分为正常对照组(n=18)，SAP组(n=18)，NAC治疗组(n=18)，各组根据6h，12h，24h时间点。重症急性胰腺炎模型参照Lankish等的方法，应用3.5%的牛磺胆酸钠(0.1mg/100g)从十二指肠对系膜缘穿刺通过胰