igf-1在mets大鼠和dm大鼠膀胱中的表达

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1、授予单位代码10089学号或申请号20123134HebeiMedicalUniversity硕士学位论文专业学位IGF-1在Mets大鼠和DM大鼠膀胱中的表达研究生：吴国柱导师：郭跃先教授专业：外科学二级学院：第三医院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独立完成。本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文主要内容相关的论文，第一署名为单位河北

2、医科大学，试验材料、原始数据、申报的专利等知识产权均归河北医科大学所有。否则，承担相应法律责任。河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写，文责自负。研究生签名：导师签章：讽年3曰目录中文摘要·······································································

3、······1英文摘要·············································································4英文缩写·············································································9研究论文IGF-1在Mets大鼠和DM大鼠膀胱中的表达前言···················································

4、··························10材料与方法····································································11结果·············································································15附图·································································

5、············18附表·············································································25讨论·············································································28结论·········································································

6、····35参考文献·······································································35综述胰岛素样生长因子-1的临床应用进展············································38致谢···················································································47个人简历············

7、·································································48中文摘要IGF-1在Mets大鼠和DM大鼠膀胱中的表达摘要目的：本实验通过建立代谢综合征和2型糖尿病大鼠模型，研究大鼠代谢综合征及2型糖尿病膀胱病变的镜下结构表现，运用免疫组化方法检测代谢综合征及2型糖尿病大鼠膀胱中胰岛素样生长因子-1（insulin-likegrowthfactor1，IGF-1）表达的变化，来探讨IGF-1在代谢综合征及糖尿病膀胱病变发生发展中

8、的作用，为进一步阻止和治疗其病变提供实验基础。方法：成年雄性Wistar大鼠19只，体重150-200g，适应性喂养1周后随机分为三组：正常对照组5只，代谢综合征组7只，2型糖尿病组7只，正常对照组予以普通饲料喂养，代谢综合征组及糖尿病组予以高糖高脂饲料（基础饲料53.5%，猪油20%，蔗糖20%，蛋黄粉25%，胆盐0.5%，及胆固醇1%组成）喂养。第8周末，测定三组动物空腹血糖、空腹胰岛素水平，正常组及代谢综合征组的空腹血脂水平，应用稳态模型评估法（HOMA-IR）=空腹血糖（F