基于meta-qtl的分子标记开发及其在种质资源评价中的应用

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1、价以及LD作图的核心标记。6．以株高的统合结果为参照系，评价了3种常规的基于QTL的标记筛选法，即直接法、剃刀法和重叠次数法。发现在剃刀法的基础上融入直接泫，其选择效果与统合分析相当，可以近乎无偏的估计统合分析给出的所有标记选取点。7．从3875个已定位的QTL中筛选出位于2H上的842个QTL进行图谱整合，对该染色体性状的整体分布进行评价，发现25．33．3、50．58．3、75．83．3、116．67．125、150．158．3cM处等5个区间同时控制着较多的性状，该位点适合选取标U对群体的整体性评价。关键词：大麦，数量性状位点，统合分析，奥坎姆剃刀律，资源评价Abstr

2、actBarley(HordeumvulgateL．)isthefourthlargestcropinChina．ItsqualitywilldirectlyaffectmeprodllctIsfinalcommercialuse．Howtoeffectivescreenoutusefulgenesfromwildandbreedingmaterialstobreedhigheryields，betterquality，greaterdiseaseresistanceandadaptabilitynewvarietiesisourcurrentobjective．Basedo

3、nlargeamountofinformationprovidedbyQTLmapping，theselectionofreason2Lblemolecularmarkerstoevaluategeneticresourcesmoreeffectivehasimportanttheoreticalandpracticalvalue．However，theinfluenceofgeneticbackgroundincrossesaIldotherfactors，suchaspopulationtype，measurementstandards，statisticalmethod

4、s，enviro珈=11ent，In{lderesultsofQTLmappingdifferently．Therefore，thepre—conditionsofexploitcoremarkerscouldensurethefacticityofQTLs．ThecoremarkerswerescreenedoutviarealQTLsthroughintegrationofQTLsfromdifferentbarleymappingpopulationsfollowedbystatisticalmethod．Themainresultsandconclusionsares

5、ummarizedasfollowing：1．Basedon150DHlinesderivedfromSteptoe×Morex，7agronomictraits，plantheight，e豺length，grainnumberperspike，1000一grainsweight，tillernumber，effectivespikeperplant，andspikenumberperplant，wereinvestigatedintwoyearatdifferentlocation．Fourpairsofpositivecorrelation(P<0．O1)werefoun

6、dintwoyears．2．150DHlineswereusedforQTLmappingandepistasisanalysiswithinclusivecomposiveintervalmappingmethod．Eventually，21QTLswerefoundforfourtraits，namely1000．grainsweight，plantheight，earlength，andgrainnumberperears·OntheoⅡlerhand，thespikenumberperplant，tillernumber，effectivespikeperplantw

7、erecontrolledmainlybyspistasiseffect．ThemarkerintervalABG399-MWG571Bwhichcontrolof4traitsonchromosome3wasstableexpressionedintwoyears．Atleast53positonalcandidategeneswerefoundinthisregionsaftermapintergration3．Throughpublishedpapers，wecollected3875QTLswh