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1、VirusResearch140(2009)179–187ContentslistsavailableatScienceDirectVirusResearchjournalhomepage:www.elsevier.com/locate/virusresAnovelmycovirusassociatedwithfourdouble-strandedRNAsaffectshostfungalgrowthinAlternariaalternataNanakoAokia,HiromitsuMoriy
2、amaa,∗,MotoichiroKodamac,TsutomuArieb,TohruTeraokab,ToshiyukiFukuharaaaLaboratoriesofMolecularandCellularBiology,DepartmentofAppliedBiologicalSciences,TokyoUniversityofAgricultureandTechnology,3-5-8Saiwaicho,Fuchu,Tokyo183-8509,JapanbLaboratoryofPlan
3、tPathology,DepartmentofAppliedBiologicalSciences,TokyoUniversityofAgricultureandTechnology,3-5-8Saiwaicho,Fuchu,Tokyo183-8509,JapancLaboratoryofPlantPathology,FacultyofAgriculture,TottoriUniversity,4-101Koyama-Minami,Tottori680-8553,Japanarticleinfoa
4、bstractArticlehistory:Fourdouble-strandedRNAs(dsRNAs),referredtoasdsRNA1(3617bp),dsRNA2(2794bp),dsRNA3Received13July2008(2576bp)anddsRNA4(1420bp),weredetectedintheEGS35-193strainofAlternariaalternataathighReceivedinrevisedformconcentration(∼3g/gdrie
5、dmycelium).Thisstrainhadanimpairedgrowthphenotype.Byexposingthe30November2008straintocycloheximideduringhyphaltipisolation,weisolatedstrainswhichhadnormalmycelialgrowthAccepted4December2008andpigmentation,inwhichdecreasedlevelsofthedsRNAswereobserved
6、(∼0.3g/gdriedmycelium).Availableonline15January2009TheseresultsindicatethatthisdsRNAmycovirusmightbeinvolvedinmodulatingtraitsofitsfungalhost,A.alternata.Thebuoyantdensityofisometricvirusparticles(about33nmindiameter)containingKeywords:thesedsRNAsin
7、CsClwas1.35–1.40g/cm3dependingonthesizeofthepackageddsRNAs.ThedsRNA1MycovirusdsRNAelementsencodesasingleopenreadingframe(3447nt)containingtheconservedmotifsofviralRNA-dependentAlternariaalternataRNApolymerase(RdRp),whichisrelatedtotheORFencodedbydsRN
8、A1ofAspergillusmycovirus341.RNA-dependentRNApolymeraseItisnoteworthythatallofthecodingstrandsofthefourdsRNAgenomeshave3-poly(A)tailsrangingVirusparticlesfrom33to50ntinlength.WenamedthisnoveldsRNAmycovirusintheEGS35-193strainA.alternatavirus-1(AaV-1)
