白藜芦醇对肠易激综合征大鼠的作用机制：涉及5-HT系统

《白藜芦醇对肠易激综合征大鼠的作用机制：涉及5-HT系统》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、温州医科大学硕士学位论文分类号：R9单位代码：10343学号：151005817硕士学位论文论文题目:白藜芦醇对肠易激综合征大鼠的作用机制：涉及5-HT系统研究生姓名：张健波学科专业：药学类型：学术型指导教师：潘建春教授二O一八年五月温州医科大学硕士学位论文论文题目：白藜芦醇对肠易激综合征大鼠的作用机制：涉及5-HT系统答辩委员会主席：刘金来主任药师答辩委员会成员：张秀华主任药师陈瑞杰主任药师郑国庆教授论文答辩日期：2018年5月28日温州医科大学硕士学位论文目录1英汉缩略语对照表·········

2、····················12中文摘要·········································23英文摘要·············································4引言·············································7正文1材料与方法···········································92结果······························

3、···················153分析与讨论·············································26参考文献···············································29附录···················································34致谢···················································35综述·············

4、······································36参考文献···············································44学位论文独创性声明·······································52温州医科大学硕士学位论文英汉缩略语对照表缩写英文中文RESResveratrol白藜芦醇IBSIrritablebowelsyndrome肠易激综合征CACSChronicacutecombiningstress慢急性

5、联合应激FSTForcedswimmingtest强迫游泳实验MBTMarble-buryingtask大理石掩埋测试AWRAbdominalwithdrawalreflex肠道腹壁撤退反射5-HT5-hydroxytryptamine5-羟色胺5-HIAA5-hydroxyindoleaceticacid5-羟吲哚乙酸(±)-8-Hydroxy-2-(±)-8-羟基-2-(二丙基8-OH-DPAT(dipropylamino)tetralinhydrobromide氨基)四氢萘氢溴酸盐NAN-19

6、0NAN-190hydrobromideNAN-190氢溴酸盐SodiumsaltofCaboxyMethylCMC-Na羧甲基纤维素钠CellulosePKAProteinkinaseA蛋白激酶AcAMP-ResponseElementBindingcAMP反应元件结合CREBProtein蛋白PhosphorylatedcAMP-Response磷酸化cAMP反应元pCREBElementBindingProtein件结合蛋白BDNFBrainderivedneurotrophicfactor脑

7、源性神经营养因子Highperformanceliquid高效液相色谱HPLC-ECDchromatography-Electrochemicaldetect-电化学1温州医科大学硕士学位论文白藜芦醇对肠易激综合征大鼠的作用机制：涉及5-HT系统中文摘要目的：观察白藜芦醇对慢急性联合应激诱导的肠易激综合征（IBS）大鼠肠道动力、感觉和心理行为的改善作用。深入研究白藜芦醇治疗IBS相关信号转导通路，探讨白藜芦醇对5-HT1A受体及5-HT-PKA-pCREB-BDNF信号通路的作用。方法：1.建立IB

8、S动物模型：采用慢急性联合应激（CACS）建立大鼠IBS模型，即CACS应激大鼠在孤笼饲养的基础上连续给予21天慢性不可预知应激，并于第22天给予3h急性束缚应激，以此建立大鼠IBS模型。2.白藜芦醇对IBS大鼠行为学的影响：采用强迫游泳实验观察动物抑郁样行为，通过大理石掩埋实验观测动物焦虑样表现，同时分别通过肠道动力学实验和腹壁撤退反射实验检测大鼠肠道动力以及内脏敏感性状态。3.观察白藜芦醇对IBS大鼠脑、肠5-羟色胺（5-HT）及其代谢产物5-羟吲哚乙酸（5-HI