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ID:34820592
大小:1.79 MB
页数:45页
时间:2019-03-11
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1、WhatisMicroarray¢Apowerfultechnologyforbiologicalexplorationwhichenablestosimultaneouslymeasuretheenablestosimultaneouslymeasurethelevelofactivityofthousandsgenes.¢TheamountofmRNAforeachgeneinagivenTheamountofmRNAforeachgeneinagivensample(orapairofsamples)ismeasured.¢Microarraysare:¢Parall
2、el¢HighHigh--throughputthroughput¢Largearge--scascallle¢GenomicscaleMostknowncommercialmicroarrayplatformsAppliedAgilentAffymetrixNembGeneIlluminaBiosystemInkjetContactMirrorPhotolithograBeads-linkedsynthesisspottiiingphlhotoliithhographyoligolibraryphy60mers25mers60mers24mers50mers¢Commer
3、cialmicroarraysgiveingeneralmorereproducibleresultsduetothhhihQCehighQC,whihihchcanbeachievedonlyatindustriallevel.RobotRobotpinspottingRobotpinspottingAggjilentinkjettechnologygy¢Agilklentusesinkjettechhnollogytoprintollligosontoglassslides.¢ThenonThenon-contactinkjettechnologyproducesmic
4、roarrayscontactinkjettechnologyproducesmicroarrayswithmoreuniformandconsistentfeatures.¢Theinkjjpetprocessdoesnotintroducedefectsasaresultofsurfacetensioninteractionswiththemicroarraysurface.¢NonNon--contactinkjetprocessprovidessubstantialcontactinkjetprocessprovidessubstantialitimprovemen
5、toverpiinspottittingwithithrespecttttoconsiisttencyandspotuniformity.Probedesign¢Proprietaryalgorithmsarealgorithmsareusedtodesigntheprobes.¢Designofconsensustargetsequencesisbasedsequencesisbasedontheintegrationofpublicdatadidbdiffderivedbydifferenttresources.¢ProbesareProbesareexperiment
6、allyvalidated.Affyyymetrixmicroarrays¢a.k.agenechip.¢Insteadofputtingentiregenesonanarray,putputsetsofDNA25putsetsofDNA25-mers(oligonucleotides)mers(oligonucleotides).¢ProducedusingaphotolithographyprocessProducedusingaphotolithography,processsimilartothatusedtomakesemiconductorchips.¢mRNA
7、samplesareprocessedseparatelymRNAsamplesareprocessedseparatelyinsteadofinpairs(singlechanneltechnology)AffyygpmetrixgeneChips¢Various25mersareputonthechiptointerrogateasinggglegene.¢Additionallyaslightvariant(thatdiffersonlyatthe13thbase)isputnexttoitbase)ispu
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