论文characterization of a novel salt tolerant bacterium gracilibacillus sp.gty and its performance of dye decolorization

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1、大连理工大学博士学位论文CharacterizationofaNovelSaltTolerantBacteriumGracilibacillussp.GTYandItsPerformanceofDyeDecolorization姓名：Md.SalahUddin申请学位级别：博士专业：环境工程指导教师：周集体20080301DalianUniversityofTechnologyDoctoralDissertationAbstractThemainaimofthisresearchworkwastodecol

2、orizevariousazodyesandreactivedyesfromlab—scaledyesolutionsandrealtextilewastewatersamplesunderhighsaltconcentrationconditions．DecolorizationexperimentswererunusingarecentlyisolatedhalophilicbacteriumGracilibaciHussp．GTY．Asanewbacterialstrain，someotherinve

3、stigations，suchasisolation，characterizationandstudyofalargeextrachromosomalDNAwerealsotakenplace．Sothedissertationispresentedindifferentstepsbellow．Firstly，ahighlysalttolerantstrainobtainedfromcoastline，WasidentifiedasGracilibacillussp．GTYonthebasisofmorph

4、ologicalandphysio—biochemicalcharacteristicsand16SrDNAsequenceanalysis．AccordingtoDNAsequence，themaximum93％-95％homologywasfoundforthestrainGTY．ThestrainwasdepositedasapatentstraininChinaGeneralMicroorganismCultureCenterwiththeaccessionnumberCGMCC1527．The16

5、SrDNAsequenceofGTYwasalsosubmittedtoGenBankwiththenumberDQ286727．Thestrainwasgram—negative，rod-shapedandstrictlyaerobic．Optimumgrowthconditionswereobserved：temperature+300C，pH7．2，10％一15％(w／v)ofNaCl，andprolineasthebestcompatiblesoluteaswellasagoodcarbonsour

6、ce．Therapidandsmoothgrowthrateswereobservedin10％一15％(w／v)ofNaCIsupplementedLuria·Bertani(LB)andMineral-Salt(MS)media，butthestraincouldsurvivenarrowlyinthemediacontainingupto25％(w／v)ofNaCl．Soitwasconsideredasahighlysalttolerantstrain．Md．SalahUddin：Character

7、izationofaNovelSaltTolerantBacteriumGracilibacillussp．GTYandItsPerformanceofDyeDecolorizationSecondly，alargemoleculeofextrachromosomalDNAwasisolatedfromthestrainbyamodifiedmethod．ThenthisplasmidDNAwaseliminatedbyacridineorangetoknowitsroleinhostgenome．Afte

8、rsuccessfulelimination，itWasassumedthattheplasmidcontainssomekeygenes，whichmayberesponsibleforthebacterialsalttolerance．Thirdly，anattemptwasmadetooptimizeconditionsforthebacterialdecolorizationofvariousdyesin