solvent tolerant marine bacterium bacillus aquimaris secreting organic solvent

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1、Chemosphere83(2011)706–712ContentslistsavailableatScienceDirectChemospherejournalhomepage:www.elsevier.com/locate/chemosphereTechnicalNoteSolventtolerantmarinebacteriumBacillusaquimarissecretingorganicsolventstablealkalinecellulaseNitinTrivedi,VishalGupta,ManojKumar,PujaKumari,C.R.K.Reddy⇑

2、,BhavanathJhaDisciplineofMarineBiotechnologyandEcology,CentralSaltandMarineChemicalsResearchInstitute,CouncilofScientificandIndustrialResearch(CSIR),Bhavnagar364021,IndiaarticleinfoabstractArticlehistory:Theorganicsolventtolerantbacteriawiththeirphysiologicalabilitiestodecontaminatetheorgan

3、icpol-Received19July2010lutantshavepotentialstosecreteextracellularenzymesofcommercialimportance.Ofthe19marinebac-Receivedinrevisedform1February2011terialisolatesexaminedfortheirsolventtoleranceat10vol.%concentration,onehadthesignificantAccepted1February2011toleranceandshowedarelativegrowth

4、yieldof86%foracetone,71%formethanol,52%forbenzene,35%forheptane,24%fortolueneand19%forethylacetate.Thephylogeneticanalysisofthisstrainusing16SrDNAsequencerevealed99%homologywithBacillusaquimaris.ThecellulaseenzymesecretedbythisKeywords:strainundernormalconditionsshowedanoptimumactivityatpH

5、11and45°C.TheenzymedidshowSolventtolerancefunctionalstabilityevenathigherpH(12)andtemperature(75°C)withresidualactivityof85%andBacillusaquimarisFattyacid95%respectively.Theenzymeactivityinthepresenceofdifferentadditiveswereinthefollowingorder:+2+2CellulaseCo>Fe>NaOCl2>CuSO4>KCl>NaCl.Theenz

6、ymestabilityinthepresenceofsolventsat20vol.%Ionicliquidsconcentrationwashighestinbenzenewith122%followedbymethanol(85%),acetone(75%),toluene(73%)andheptane(42%).Thepre-incubationofenzymeinionicliquidssuchas1-ethyl-3-methylimida-zoliummethanesulfonateand1-ethyl-3-methylimidazoliumbromideinc

7、reaseditsactivityto150%and155%respectively.Thechangeinfattyacidprofilewithdifferentsolventsfurtherelucidatedthephysio-logicaladaptationsofthestraintotoleratesuchextremeconditions.Ó2011ElsevierLtd.Allrightsreserved.1.Introductionnizingthecellmembraneandfinallycau