清肝益气降压方对自发性高血压大鼠血管重构干预机制

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1、提要目的：通过观察清肝益气降压方对自发性高血压大鼠(SHR)血压、心率、血生化指标和高血压调控因子等因素的影响，探讨本方对血管重构的干预机制，为原发性高血压的治疗寻求有效治法和方药。方法：采用国际通用自发性高血压大鼠病理模型灌胃给药，观察本方的降压作用：检测ET、NO、AngII等指标；通过对SHR肾常规切片进行HE染色和Masson染色，用光学显微镜观察本方对SHR肾内小动脉组织形态学的影响。运用免疫组化技术和原位杂交技术，观察本方对SHR肾内小动脉内皮型一氧化氮合酶基因和血管紧张素II1型受体基因表达的影响。结果

2、：本方有确切的降压作用，给药后与模型对照组相比血压明显下降(P

3、pertensiveFormulaofClearingLiverFireandSuppiementingQiandInterferentialMechanismSpecialty：FormulaeofTraditionalChineseMedicineAuthor：GnoWeiTutor：ProfessorHanTaoAbstractobiective：Toobservetheeffectofantihypertensiveformulaofcleatingliverfireandsupplementingqionb

4、loodpressure，cardiotaeh，bloodbiochemistryindexsignandmodulatorsofspontaneouslyhypertensiverat(SHR)，theauthorwantstodiscusstheinterferentialmechanismonvascularremodelinginSHR，toseektheeffecovetherapeuticmethodtotreatessentialhypertension．Methods：Adoptinginternat

5、ionallySHRaspathologicalmodel，givendrugsbyinspiregastric，ET、AngIIinplasmaandNOinsel'umweredetected．CuttingkidneyofSHRintoslicesroutinely,anddyeingthetissuesofthosewithHEandMasson，theauthorobservestheeffectonthearteriolarinkidneywiththeopticalmicroscope．Managing

6、immunocytochemistrytechniquesandinsituhybridization，theauthorobservesitseffectontheexpressonofeNOSgeneandATlRgeneofthearteriolarinkidney．Results：Theformulahasassuredantihypertensiveeffect．withmakingthebloodpressureobviouslydropcomparedwiththemodel(P

7、improveNOlevelinserumandadjustthebalanceofET／NO，controlcontractingofbloodvessel，reducetheplasmaAngIIandhasgoodpreventiveeffectonthefibrosisofbloodvesselofSHR．Conclusions：Theformulaofclearingliverfireandsupplementingqihasgoodantihypertensiveeffect，combinationofd

8、ragsofclearingliver,supplementingQi，tonifyingYin，soothingthelivercanstrengthenitsantihypertensiveeffectonSHR，Huangqi(AstragalusRoot)hasobviouscompatibilityenlargementutility