草鱼呼肠孤病毒II型毒株分离及TaqMan Real-time PCR快速检测方法的建立

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1、HUAZHONGAGRICULTURALUNIVERSITY硕士学位论文MASTER’SDEGREEDISSERTATION草鱼呼肠孤病毒II型毒株分离及TaqManReal-timePCR快速检测方法的建立ISOLATIONOFGRASSCARPREOVIRUSGENOTYPEIISTRAINANDTHEESTABLISHMENTOFTAQMANREAL-TIMEPCRMETHODFORDETECTINGIT研究生:刘欣苑CANDIDATE：LIUXINYUAN学号:2016308120002STUDENTNO.：专业:农业硕士MAJOR：MAST

2、EROFAGRICULTURER导师:刘学芹教授SUPERVISOR：PROFESSORLIUXUEQIN中国武汉WUHAN，CHINA二○一八年六月JUNE，2018华中农业大学硕士学位论文草鱼呼肠孤病毒II型毒株分离及TaqManReal-timePCR快速检测方法的建立IsolationofGrassCarpReovirusGenotypeIIStrainAndTheEstablishmentofTaqManReal-timePCRMethodforDetectingIt研究生:刘欣苑学号:2016308120002指导教师:刘学芹教授学位类型

3、:农业硕士领域：渔业华中农业大学水产学院中国·武汉CollegeofFisheriesHuazhongAgruiculturalUniversityWuhan，China草鱼呼肠孤病毒II型毒株分离及TaqManReal-timePCR快速检测方法的建立目录摘要...................................................................................................................................................

4、iAbstract...........................................................................................................................................iii第一章文献综述........................................................................................................................

5、.....1前言..................................................................................................................................................11草鱼呼肠孤病毒的国内外研究进展...........................................................................................11.1草鱼出血病与草鱼呼肠孤病

6、毒........................................................................................11.2病毒结构、形态及理化特征...........................................................................................21.3细胞培养特性........................................................................

7、...........................................31.4草鱼呼肠孤病毒基因分型的相关研究...........................................................................41.5GCRV基因组结构和功能分析.......................................................................................51.6GCRV的检测方法......................

8、......................................................