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1、第21卷第4期高校化学工程学报No.4Vol.212007年8月JournalofChemicalEngineeringofChineseUniversitiesAug.2007文章编号：1003-9015(2007)04-0685-04强光照对雨生红球藻合成虾青素的影响1,212董庆霖,赵学明,邢向英(1.天津大学化工学院,天津300072;2.河北工业大学化工学院,天津300130)摘要：在不同的光照强度下研究了雨生红球藻细胞内虾青素的合成与初级代谢的关系。在强光(HL)和中等强度(ML)的光照条件下，雨生红球藻细胞内1,5-二磷酸核酮糖羧化酶(
2、Rubisco)和硝酸还原酶(NR)的活性第1天大幅度提高，2天后又迅速下降。与此同时，硝酸盐浓度也快速降低。当虾青素在第4天(HL)和第6天(ML)开始合成时，HL中Rubisco−和NR活性以及NO3浓度分别下降了75.5%，71.5%和96.2%，而ML中则下降了76.5%，74.7%和94.3%。相比之下，在低光照(LL)条件下，实验结束时三个指标仅下降了25.9%，29.8%和56.8%，细胞中没有虾青素积累。结果表明强光提高了Rubisco和NR活性，导致硝酸盐浓度迅速降低而最终又抑制了这两种酶的活性，造成雨生红球藻光合作用效率下降即“碳
3、饥饿”。在此状态下，为了生存，细胞内合成虾青素的相关基因被激活，藻细胞开始合成并积累虾青素。关键词：雨生红球藻；虾青素；强光；硝酸还原酶；1,5-二磷酸核酮糖羧化酶中图分类号：Q949.212文献标识码：AEffectofHighIrradianceonAstaxanthinSynthesisinHaematococcuspluvialis1,212DONGQing-lin,ZHAOXue-ming,XINGXiang-ying(1.SchoolofChemicalEngineering,TianjinUniversity,Tanjin300072,
4、China;2.SchoolofChemicalEngineering,HebeiUniversityofTechnology,Tanjin300130,China)Abstract:TherelationshipbetweenastaxanthinsynthesisandprimarymetabolisminHaematococcuspluvialiswasstudiedunderdifferentintensitiesoflight.Theresultsshowthattheactivitiesofribulose-1,5-bisphosphat
5、ecarboxylase(Rubisco)andnitratereductase(NR)increasesignificantlyonthefirstdayunderhigh(HL)andmedium(ML)lightintensities,andfalldrasticallyaftertwodays.Atthesametime,nitrateconcentrationalsodecreasesmarkedly.Whenastaxanthinsynthesiswastriggeredon4th(underHL)and6th(underML)days,
6、RubiscoandNRactivitiesaswellasnitrateconcentrationdecline75.5%,71.5%,96.2%underHLand76.5%,74.7%,94.3%underML,respectively.Ontheotherhand,underlowlightintensity(LL),thesethreeparametersdecreaseonly25.9%,29.8%and56.8%attheendoftheexperimentandwithnoastaxanthinaccumulatedinthealga
7、lcells.TheseresultssuggestthatthehighirradiancestimulatestheactivitiesofRubiscoandNR,whichleadstorapiddecreaseofnitrateconcentrationandultimatelyinhibitstheactivitiedofthesetwoenzymes,andconsequentlyH.pluvialisbecomes“carbonstarvation”intermsofphotosynthesis.Forsurvival,theasta
8、xanthin-relatedgenesareactivated,andthealgalcellsbegin
