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1、文章编号:10002677X(2010)0120062204体育科学2010年(第30卷)第1期CHINASPORTSCIENCEVol.30,No.1,62265,2010.不同方式游泳训练对大鼠纹状体cAMP浓度影响的实验研究EffectofwDifferentStyleSwimmingExerciseoncAMPContentsinStriatumofRats12341张安民,张青元,于芳,胡淑萍,李晓旭12341ZHANGAn2min,ZHANGQing2yuan,YUFang,HUShu2ping,LIXiao2xu摘要:目的:观察持续游泳训练和间歇性负重游泳训练后大鼠纹状体cA
2、MP浓度变化。方法:雄性SD大鼠55只,随机分为对照组、持续训练组、间歇训练组。建立大鼠持续游泳和间歇性负重游泳训练模型,采用大鼠环磷酸腺苷cAMPELISA法,观察大鼠纹状体cAMP浓度变化,并对之进行统计分析。结果:训练后,除间歇组0.5hcAMP值显著低于对照组外(P<0.01),不同训练组各时刻大鼠纹状体cAMP浓度均高于对照组(P<0.01);持续训练后,大鼠纹状体cAMP浓度逐步升高,至0.5h时达到最高值,其后逐渐下降,至4h时仍高于对照组水平,其浓度变化曲线呈先升后降特征;间歇训练后,大鼠纹状体cAMP浓度较对照组明显升高,之后0.5h时急剧下降。1h时cAMP浓度值达到最
3、高值;2h时cAMP浓度较1h时显著下降,但仍高于对照组水平;4h时其浓度又有所升高,整个浓度变化曲线呈锯齿形。结论:cAMP信号系统密切参与纹状体训练调节过程;持续游泳训练后纹状体cAMP浓度变化呈先升后降特征;间歇性负重游泳训练后纹状体cAMP浓度变化呈反复升降特征。关键词:纹状体;cAMP;游泳;鼠;动物实验Abstract:Objective:ToobservethecAMPcontentsinstriatumofratsindifferentmomentaf2terenduranceswimmingandintermittentloadswimming.Methods:55mal
4、eSDratswereran2domlydividedintothecontrolgroup,enduranceswimmingandintermittentswimminggroup.Toestablishtheenduranceswimmingandintermittentswimmingmodelsinrat,anddetectthecAMPcontentsinstriatumofratsinstriatumbycAMPELISAmethods.Thedataisstatisti2callyanalyzed.Result:1)Afterexercise,thecAMPcontents
5、instriatumofratsindifferentmomentafterenduranceswimminghashigherlevelthancontrolgroup(P<0.01),besidesthecAMPcontentsoftheintermittentgroupat0.5hislowerthanthecontrolgroup(P<0.01);2)Instriatum,thecAMPcontentsincreaseslightlyandreachthepeakat0.5h,thendecreasegradually,butthelevelisstillhigherthantha
6、tofcontrolgroupat4hafterswimming.ThechangefigureofcAMPcontentsappearsthecharacteroffirstlyrisingthendroppingmoderate2lyafterenduranceswimming;3)ThecAMPcontentsinstriatumofratsindifferentmomentafterintermittentswimminghashigherlevelthancontrolgroup(P<0.01)butat0.5hwhichhaslowerlevelthancontrolgroup
7、(P<0.01).Afterthat,thecAMPcontentsrisingrapidlyandreachthepeakat1hafterswimming,thenthecAMPcontentsdrops,at2hafterswim2ming,thelevelwasstillhigherthanthatofcontrolgroup.ThecAMPcontentsincreaseafteralittle.Thechan
