山药块茎发育中物质积累及相关代谢酶变化

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1、作物学报ACTAAGRONOMICASINICA2011,37(5):903910http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2011.00903山药块茎发育中物质积累及相关代谢酶变化1111,*222梁任繁李创珍张娟何龙飞韦本辉甘秀芹何虎翼12广西大学农学院,广西南宁530004;广西农业科学院经济作物研究所,广西南宁530007摘要:以桂淮5号和桂淮16两个不同山药品种为材料,根据块茎生长发育特性,在山药

2、发育进程中测定块茎的有关生理生化指标。结果表明,随着块茎发育进程的推进,块茎长度及干物质积累持续增加,淀粉酶(AMY)活性降低;多数碳水化合物、功能性物质及过氧化物酶(POD)、酸性转化酶(AI)和蔗糖磷酸合酶(SPS)活性先持续增加,达到峰值后下降,但峰期不同;2个品种的还原糖、ADPG焦磷酸化酶(AGPase)变化不一致。t检验表明,在块茎发育全过程中,****品种间的干物质(P=0.011)、淀粉(P=0.031)、皂苷(P=0.019)差异显著。可溶性糖(r5=0.97,r16=1.00)、AGPase(r5=*******0.85)、还原糖(r16=0.87)与蔗糖显著

3、相关;多糖(r5=0.95)、Vc(r5=0.83)、皂苷(r16=0.88)与淀粉显著相关;干物质********(r5=0.97,r16=0.87)、AMY(r16=0.95)、AGPase(r16=0.90)、蛋白质(r16=0.83)与块茎伸长膨大显著相关。由此表明,蔗糖积累、SPS和AI活性在山药块茎发育中起关键调控作用,主要功能性物质彼此间密切相关。关键词:山药;块茎发育;物质积累;酶ChangesofMatterAccumulationandRelativeEnzymaticActivityduringYamTuberDevelopment1111,*22LIAN

4、GRen-Fan,LIChuang-Zhen,ZHANGJuan,HELong-Fei,WEIBen-Hui,GANXiu-Qin,andHE2Hu-Yi12CollegeofAgronomy,GuangxiUniversity,Nanning530004,China;InstituteofEconomicCrops,GuangxiAcademyofAgriculturalSciences,Nanning530007,ChinaAbstract:Tuberdevelopmentisacomplexphysiologicalprocess,involvinginchangesofva

5、riouscomponentsandenzymesac-tivities,solearningaboutchangesofthematteraccumulationandenzymesmetabolismwillhelptoinstructyamproduction.Inthepaper,twodifferentcultivarsGuihuai5andGuihuai16wereusedtoinvestigatesomephysiologicalandbiochemicalindica-torsduringyamtuberdevelopmentin2009—2010.Theresul

6、tsshowedthattuberlengthanddrymattercontentwerekeptin-creasing,butAMYenzymeactivitydecreasingduringthetuberdevelopment.Themaincarbohydratescontents,functionalsub-stances,andtheactivitiesofPOD,AI,andSPSpresentedsimilartrend:continuallyincreasedfirstly,andreachedvariouspeaks,thendecreased.Thechan

7、gesofreducingsugarcontentandAGPaseactivityweredifferentbetweentwocultivars.Thet-testre-sultsindicatedthattherewasasignificantdifferencebetweentwocultivarsindrymatter(P=0.011),starch(P=0.031),andsaponin****(P=0.019).Sucrosecontentwassign