人细小病毒b19-xa2株vp1蛋白bac-to-bac系统表达及免疫学特性分析

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1、独创性声明秉承学校严谨的学风与优良的科学道德，本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，不包含本人或他人已申请学位或其他用途使用过的成果。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了致谢。申请学位论文与资料若有不实之处，本人承担一切相关责任。论文作者签名：日期：保护知识产权声明本人完全了解第四军医大学有关保护知识产权的规定，即：研究生在校攻读学位期间论文工作的知识产权单位属第四军医大学。本人保证毕业离校

2、后，发表论文或使用论文工作成果时署名单位仍然为第四军医大学。学校可以公布论文的全部或部分内容（含电子版，保密内容除外），可以采用影印，缩印或其他复制手段保存论文。学校有权允许论文被查阅和借阅，并在校园网上提供论文内容的浏览和下载服务。论文作者签名：导师签名：日期：人细小病毒B19-XA2株VP1蛋白Bac-to-Bac系统表达及免疫学特性分析研究生：李小青学科专业：儿科学所在单位：第四军医大学导师：张国成教授（主任医师）辅导教师：许东亮副主任技师资助基金项目：国家自然科学基金资助项目（30571948）关键词：人细小病毒B19；VP1蛋白；原核表达；穿

3、梭载体；真核表达；基因转染；免疫原性；反应原性中国人民解放军第四军医大学2008年05月第四军医大学博士学位论文目录缩略语表··············································································································1中文摘要······································································································

4、········4英文摘要··············································································································8前言············································································································14文献回顾·······································

5、·····································································16正文人细小病毒B19-XA2株VP1蛋白BAC-TO-BAC系统表达及免疫学特性分析····················································································34实验一人细小病毒B19-XA2株VP1基因原核表达载体的构建、重组蛋白表达及初步鉴定······································

6、·······································341材料·········································································································341.1主要仪器···························································································341.2主要材料与试剂··················

7、·····························································351.3主要工作液的配制···········································································372方法·········································································································402.1人细小病毒B19-XA2株VP

8、1基因原核表达载体的构建与鉴定·······402.2人细小病毒B19-XA2株VP1蛋白原核