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1、5cm.‘Maddy’and‘Fairy’wereclassifiedintoasubgroupwhichwereredcolored.2Pollensof10RosahybridaminimacultivarswereexaminedbyLM(LightMicroscopy)andSEM(ScanningElectronMicroscopy).Theclusteranalysiswascarriedoutbasedupontheobservationresults.Thepollengrainsweretricalporateand3-lobedcircula
2、rinpolarview.Differentcultivarsshoweddifferentexinesculptureandfringes，luminasandverrucate.Thepollenshapeswerespheroidalorsuper-spheroidalbyP/E(Polaraxislength/Equatorialaxislength)criteria.Theresultofclusteranalysisindicatedthatthecultivarscouldbeclassifiedintothreegroupswhensimilar
3、itycoefficientwas0.37.Thefirstgroupincluded‘WhiteKoster’,‘PinkKoster’,‘PurpleMinistar’,‘Romantic’.Thesecondgroupincluded‘GoldSun’,‘Maddy’,‘DwarfFairy’,‘HulaGirl’,‘WinterPlum’.Thethirdgroupincluded‘RedCloud’.325Rosahybridaminimacultivarswereusedforgeneticrelationshipanalysisbyusingsim
4、plesequencerepeat(SSR).46alleleswereamplifiedbyusing16pairsofprimersselectedfrom23pairs,ofwhichaverage2.9allelesperpairofprimerwereamplifiedandcalculationofthesimilaritycoefficientandalsoclusteranalysisshowedthat:ThevariancerangeofgeneticsimilaritycoefficientofSSRmarkersamongthevarie
5、tieswere0.4565-0.9783,andthegneticdistancewere0-0.9262.Thecultivarscouldbeclassifiedintothe2groupswhensimilaritycoefficientwas0.58.Thefirstgroupincluded‘WinterPlum’and‘PurpleMinistar’andthesecondgroupincluded‘Charlesself-expenser’，‘BlackJade’,‘DwarfFairy’,‘Carbuncle’,‘Purpletimes’,‘G
6、oldSun’,‘Strawberryswirl’,‘RebirthGlacier’,‘Razzle’,‘Clarissa’,‘BluePitter’,‘SweetDream’,‘Snow’,‘WhiteKoster’,‘PinkKoster’,‘Whitepearl’,‘Korean’,‘Maddy’,‘Freegold’,‘Rainbow’,‘Sunmaid’,‘Smallwelfare’and‘RedCloud’.4TheMorphology,PalynologyandSSRanalysesclassificationresultsareproximate
7、lysame，buttherewerealsosomedifferences.‘WhiteKoster’,‘PinkKoster’,‘Korean’and‘RedCloud’wereclassifiedintoonegroupbothbymorphologicalclassificationandSSRtechnique,whichshowedtherewascloserrelationshipsbetweenthem.‘PurpleMinistar’,‘WhiteKoster’and‘PinkKoster’wereclassifiedintoagroupbym
8、orphological
