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1、5cm.‘Maddy’and‘Fairy’wereclassifiedintoasubgroupwhichwereredcolored.2Pollensof10RosahybridaminimacultivarswereexaminedbyLM(LightMicroscopy)andSEM(ScanningElectronMicroscopy).Theclusteranalysiswascarriedoutbasedupontheobservationresults.Thepollengrainsweretricalpo
2、rateand3-lobedcircularinpolarview.Differentcultivarsshoweddifferentexinesculptureandfringes，luminasandverrucate.Thepollenshapeswerespheroidalorsuper-spheroidalbyP/E(Polaraxislength/Equatorialaxislength)criteria.Theresultofclusteranalysisindicatedthatthecultivarsc
3、ouldbeclassifiedintothreegroupswhensimilaritycoefficientwas0.37.Thefirstgroupincluded‘WhiteKoster’,‘PinkKoster’,‘PurpleMinistar’,‘Romantic’.Thesecondgroupincluded‘GoldSun’,‘Maddy’,‘DwarfFairy’,‘HulaGirl’,‘WinterPlum’.Thethirdgroupincluded‘RedCloud’.325Rosahybrida
4、minimacultivarswereusedforgeneticrelationshipanalysisbyusingsimplesequencerepeat(SSR).46alleleswereamplifiedbyusing16pairsofprimersselectedfrom23pairs,ofwhichaverage2.9allelesperpairofprimerwereamplifiedandcalculationofthesimilaritycoefficientandalsoclusteranalys
5、isshowedthat:ThevariancerangeofgeneticsimilaritycoefficientofSSRmarkersamongthevarietieswere0.4565-0.9783,andthegneticdistancewere0-0.9262.Thecultivarscouldbeclassifiedintothe2groupswhensimilaritycoefficientwas0.58.Thefirstgroupincluded‘WinterPlum’and‘PurpleMinis
6、tar’andthesecondgroupincluded‘Charlesself-expenser’，‘BlackJade’,‘DwarfFairy’,‘Carbuncle’,‘Purpletimes’,‘GoldSun’,‘Strawberryswirl’,‘RebirthGlacier’,‘Razzle’,‘Clarissa’,‘BluePitter’,‘SweetDream’,‘Snow’,‘WhiteKoster’,‘PinkKoster’,‘Whitepearl’,‘Korean’,‘Maddy’,‘Free
7、gold’,‘Rainbow’,‘Sunmaid’,‘Smallwelfare’and‘RedCloud’.4TheMorphology,PalynologyandSSRanalysesclassificationresultsareproximatelysame，buttherewerealsosomedifferences.‘WhiteKoster’,‘PinkKoster’,‘Korean’and‘RedCloud’wereclassifiedintoonegroupbothbymorphologicalclass
8、ificationandSSRtechnique,whichshowedtherewascloserrelationshipsbetweenthem.‘PurpleMinistar’,‘WhiteKoster’and‘PinkKoster’wereclassifiedintoagroupbymorphological
