柑橘类植物erf6和gras基因的低温胁迫应答与启动子序列分析

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1、COLDSTRESSRESPONSEOFE腑AND删SGENEINSOⅣ匝CITRUSPLANTSANDTHEIRPROMOTERSEQUENCEANALYSISABSTRACTThelowtemperatureisoneofthemajorenvironmentalfactorsaffecttheyieldandqualityofeconomiccrops．Citrusplants(Citrus)isoneoftheimportanteconomiccropsinChina,whichhasafar-r

2、angingusesas舶shfruitsormedicinalplants．Citurshasaverylargeacreageinourcountry．However,asasubtropicalplants、析tllpoorresistanceofcold，thecultivationrangeofCiturshasbeenseriouslyhamperedbythecyclicalfrozendisasterofplants．Peoplehavebeendedicatedtousetransgen

3、icbreedingtoimprovethecoldresistanceofthoseplants．Thetranscriptionfactorproteinsplayanimportantroleintheplantabioticstressresponses．ERFandGRAS,twotranscriptionfactorsspecificinplant，arereportedtobeinvovedinaboveprocess．ThecitrusplantFingeredcitron(C．medic

4、aCV．Qingpi)，BenDiZao(CreticulataCV．Succosa)，OuGan(CreticulataCV．Suavissima)andChuHongYou(CmaximaCV．chuhongyou)arevariousatcoldresistance．Poncirus(PoncirustrifoliateRaf．)iscloserelatedtoCitruswhile、析吐lahighcoldresistance．Westudiedtherelationshipbetweenthed

5、ifferentexpressionsoftranscriptionfactorERF6andGRASandthecoldresistancestrengthin2yearsoldFingeredcitron,BenDiZao，OuGart,ChuHongYouandPoncirus，andthenweclonedthepromotersequenceofERF6andGRASgeneinFingeredcitronandpreliminaryanalyzedtheirtranscriptionalIIl

6、regulationmechanism．1．Wreselected2yearsoldFingeredcitron,BenDiZao，OuGan,ChuHongYouandPoncirusasexperimentalmaterialstotestetherelativeelectricconductivity(REC)inleavesofthesefivespecies，andthenanalyzedthecoldresistancetocalculatetheirsemilethaltemperature

7、s(L1’50)aftertreatingaboveplantsunderaseriesoflowtemperaturesfor24h．111econtentofthemalondialdehyde0VIDA)ofthefivespeciesweremesuredandthecoldresistancestrengtheswerecomparedaftertheplantsunder4℃treatmentedforvariousofhours．，nleresultsindicatedthattheRECo

8、fFingeredcitronandotherfourplantsincreasedatvariousratio、舫廿lthetemperaturedrop，allofthemshowinganS-curveshape；111eLT50ofFingeredcitron,BenDiZao，OuGan,ChuHongYouandPonciruswere一5．2。C，-8．6"C，一8．3。C，一6．6"Cand-10．0"C，re