补肾对超排卵周期颗粒细胞gdnf、gfr_α-1表达的影响

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1、结论：1.获得与既往国家级和省级课题相同的临床妊娠率（54%左右）。在IVF-ET治疗周期，应用补肾中药可明显改善患者肾虚症状，减少Gn用量，提高优质卵率、优质胚胎率，进而改善临床妊娠率，是临床常用的有效药物。2.补肾中药提高颗粒细胞GDNF、GFR-1mRNA的表达，改善颗粒细胞分泌功能，这可能是补肾中药改善IVF结局的机制之一。3.补肾中药促进小鼠GV期卵母细胞生殖泡的破裂（GVBD）和第一极体（PB1）的排出；提高小鼠卵母细胞GFR-1mRNA的表达，并改善了小鼠受精卵的卵裂、形成囊胚的能力。推断：补肾中药提高颗粒细胞GDNF、GFR-

2、1mRNA的表达，提高小鼠卵母细胞GFR-1mRNA的表达，促进卵母细胞GVBD和PB1的排出，从而改善了卵母细胞成熟度，提高卵细胞质量，从而提高胚胎质量及临床妊娠率。这可能又是补肾中药改善IVF结局的机制之一。关键词补肾；肾主生殖；控制性超排卵；颗粒细胞GDNF、GFR-1mRNA1EffectsofReinforcingKidneyontheExpressionofGDNF、GFR-linGranulosaCellofSuperovulationCycleSpeciality:GynecologyofTCMAuthor：CHENYan-h

3、uaTutor：Prof.LIANFangAbstractObjective：ToinvestigatetheeffectsofreinforcingkidneyontheexpressionofGDNF、GFR-lingranulosacellofsuperovulationcycle,InviewofGVBD、extrusionofPB1andoocytematurationparacrine,toexplorethemechanismofreinforcingKidneyChineseherbsErzhitiangui(ETG)Granul

4、etoimprovethetreatmentoutcomeofinsuperovulationcycle.Method：ClinicalStudy:80infertilewomenwithtubalfactorundergoingvitrofertilization-embryotransfer(IVF-ET)wererandomizedintothestudygroup,treatedwithETGandGn(40cases),andthecontrolgroup,treatedwithplacebosgranulesandGn(40cases)

5、.Thelongtermprotocolwasadopted.TheE2andPsecretioningranulecellafterculturedfor24h、48hinvitrowasobservedtolearnaboutthesecretingfunctionofgranulecell.TheexpressionofGDNF、GFR-1mRNAinluteinizinggranulosacellswerealsodetected.ThesyndromescoresofTCM,administrationtimeandnumberofGn

6、,numberofretrievedoocytes,fertilizationrate,highqualityembryorateandpregnancyratewereevaluated.ExperimentalStudy:165Kunmingfemalemiceof8-9weeksoldwereallocatedintothreegroupsrandomly:controlgroup(groupHMG＋HCG＋NS),HMGandHCGweregivenforovarianstimulation,performedabdomeninjectio

7、nwith0.9%saltsolutionofsameasstudygrouponthesametimeschedule;studygroup(groupHMG＋HCG＋ETG);ETGcombinedwithHMGandHCGweregiven;blankgroup(groupnothing).Threegroupweredividedinto1、2groupseach.1groupweretakeddenudedongerminalvesiclestage，observedthegerminalveiclesbreakdowmandextrus

8、ionofthefirstpolarbody,TheexpressionofGDNF、GFR-1mRNAintheooc