中国汉族冠心病患者乙醇脱氢酶2基因多态性与2型糖尿病的相关性研究

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1、山东人学硕士学位论文pcctofis154casesandchronicstablecoronaryheartdisease20cases．Thesubjectscomprised63femalesand164males，、Ⅳithmeanage61．7±10．4years．ItsclinicalmanifestationsandECGconformedtothe1979WHOdiagnosticcriteriaforcoronaryheartdisease，晰matleastonenarrow,

2、and三50％(diameter)bycoronaryangiography．Take2mlarterialbloodwithsodiumcitrateanticoagulation．AbstractDNAfromtheblood．DesignspecificprimersforthetargetDNAfragments，thengoonPCR．Electrophoresisamplifiedproductsinthe2％agarosegel。dividethesubjcotsintothewil

3、d-typegroupandthemutantgroupaccordingtoelectrophoresisresults．Inquireandcollectthesubjects’detailedinformationaboutthedisease：age，sex，hypertension,T2DM，familyhistoryofcardiovasculardisease，face-reactionafterdrink，smoking，historyofalcohol，waistcircumfe

4、renceandotherbasicinformation．Takeearlymorningblood(fastingfor10hours)，thenmeasurefastingglucose，glycosylatedhemoglobin(HbAlc)，totalcholesterol(TO，triglycerides(TG)，high-densitylipoprotein(HDL—O，low-densitylipoprotein(LDL—C)；accountthealcoholvolumnthr

5、oughinquisition．UseSPSS11．5statisticalsoftwarepackagesfordataanalysis，x士sfornumericalvariablesandfrequencyforclassificationvariables．t-testareusedincomparisonofnumericalvariablesandX2inclassificationvariables．Usemulti-factorlogisticregressiontocorrect

6、eage，hypertension，TGsmoking，familyhistoryofcardiovasculardiseaseandotherfactors．ApproachtherelationshipbetweenADH2genotypesinCHDpatientsandtheT2DMprevalence．ThatP<0．05showsastatisticalsignificance．ResuiIs21．1percentofthesubjectshaveahistoryoftype2diab

7、etes．ThenumberofCHDpatientswithwild-typegroupofADH2genesis102cases，and谢thmutant-typegroupis125cases．39．2percentofpatientswithCHDinthewild·typegrouphaveT2DM，significantlyhigherthan6％ofthemutate—typegroup(尸

8、hatADH2genotypeandT2DMweremorerelevant(尸<0．01)．ForsubjectswithoutahistoryofT2DM,thelevelofBMI，waistcircumference，6山东大学硕七学位论文serumtotalcholesterol，LDL·C，triglycerideincreasesaSHbAlcquartilenumemsincreases．Logisticmultipleregressionanalysisshows