资源描述：
《红树林放线菌的分离及其抗菌和抗肿瘤细胞活性》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、应用与环境生物学报2008,14(2):244~248ChinJApplEnvironBiol=ISSN1006-687X2008-04-25*红树林放线菌的分离及其抗菌和抗肿瘤细胞活性1,222212**肖静许静谢庶洁张熙颖喻子牛徐俊(1华中农业大学生命科学技术学院农业微生物学国家重点实验室武汉430070)(2国家海洋局第三海洋研究所海洋生物遗传资源重点实验室福建厦门361005)摘要从福建漳州红树林保护区采集土壤样品中分离到163株放线菌,用5种指示菌(Bacillussubtilis,Staphylococ-cusaureus
2、,Escherichiacoli,CandidaalbicansandRhizoctoniasolani)测定了它们的抗菌活性,结果表明,其中4213%的放线菌发酵液具有单一或多种抗菌活性.同时测定了它们的发酵液对肝癌细胞BEL7402、肺癌细胞A549和白血病细胞HL60三种肿瘤细胞的毒性,其中3714%的放线菌发酵液具有单一或多种抗肿瘤细胞毒活性.对这163株放线菌的16SrDNA序列分析表明,它们分属于链霉菌属(89%)、小单孢菌属(611%)、糖单孢菌属(016%)、马杜拉放线菌属(317%)和拟诺卡氏菌属(016%).其中,
3、3株链霉菌与其亲缘关系最近的菌种的16SrDNA相似性低于97%,可能是3个新种.图3参15关键词放线菌;红树林;抗菌活性;抗肿瘤细胞活性;16SrDNACLCQ939.130.6*IsolationofMangroveActinomycetesandTheirAntagonisticActivities1,222212**XIAOJing,XUJing,XIEShujie,ZHANGXiying,YUZiniu&XUJun(1StateKeyLaboratoryofAgricultureMicrobiology,CollegeofL
4、ifeSciences,HuazhongAgricultureUniversity,Wuhan430070,China)(2KeyLaboratoryofMarineBiogeneticResources,TheThirdInstituteofOceanography,StateOceanicAdministration,Xiamen361005,Fujian,China)Abstract163actinomycetesstrainswereisolatedfrommangrovesedimentsinZhangzhou,Fujian
5、,China.Fiveindicatorstrains(Bacillussubtilis,Staphylococcusaureus,Escherichiacoli,Candidaalbicans,Rhizoctoniasolani)andthreetumorcells(BEL7402,A549andHL60)wereusedtotesttheant-imicrobialandant-itumoractivitiesoftheisolatedstrains.4213%ofthefermentationbrothoftheactinomy
6、cetesshowedantimicrobialactivities,while3714%ofthefermentationbrothofactinomy-cetespossessedant-itumoractivities.16SrDNAsequenceanalysisoftheactinomycetesstrainsshowedthattheybelongtoStrep-tomyces(89%),Micromo-nospora(6.1%),Sacharomonospora(016%),Actinomadura(317%)andNo
7、cardiopsis(016%).ThreeisolatesweresuggestedasnovelspeciesofStreptomycesbasedontheir16SrDNAsequences.Thehighest16SrDNAsim-ilaritycouldbefoundineachblastsearchanditwaslowerthan97%.Fig3,Ref15Keywordsactinomycetes;mangrovesediment;ant-imicrobialactivity;ant-itumouractivity;
8、16SrDNACLCQ939.130.6放线菌是原核生物中高G+C含量的革兰氏阳性细菌类菌和3种肿瘤细胞作为模型,测定了所获菌株的抗菌和抗肿瘤群,在自然界中分布很广,不仅在自然界物质能量循环中扮演细胞活性;并对分离得到的
