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1、北京大学学报(医学版)402JOURNALOFPEKINGUNIVERSITY(HEALTHSCIENCES)Vo.l38No.4Aug.2006论著吸入异氟烷对大鼠肺表面活性蛋白A的影响李志红,杨拔贤,安海燕(北京大学人民医院麻醉科,北京100044)[摘要]目的:观察大鼠在吸入不同浓度异氟烷后,肺表面活性蛋白A(surfactantproteinA,SPA)的含量及其基因表达的改变。方法:雄性Wistar大鼠32只随机分为对照组(Control),0.7%(体积分数)异氟烷组,1.5%异氟烷组和
2、2.0%异氟烷组,每组8只。各组分别吸入40%(体积分数)O2和设定浓度的异氟烷8h,对照组只吸入40%O8h,然后迅速处死大鼠。取肺组织用透射电镜观察肺泡!型上皮细胞结构变化。检测支气管肺泡灌洗液中和2!型上皮细胞中SPA的含量以及肺组织中SPAmRNA的表达。结果:吸入异氟烷后,1.5%异氟烷组和2.0%异氟烷组的肺泡!型上皮细胞可见明显形态学改变。各吸入异氟烷组的支气管肺泡灌洗液中SPA的含量均较对照组显著下降(Control组,437112∀25654;0.7%异氟烷组,355789∀28116;1.5
3、%异氟烷组,238554∀31531;2.0%异氟烷组,223632∀25710;P<0.01);1.5%异氟烷组和2.0%异氟烷组较0.7%异氟烷组下降更明显(P<0.01)。免疫组化显示:1.5%异氟烷组与2.0%异氟烷组与对照组肺泡!型上皮细胞中SPA的含量明显下降,而0.7%异氟烷组较对照组无明显变化。吸入异氟烷的各组SPAmRNA的表达均有降低趋势,1.5%异氟烷与2.0%异氟烷组与对照组比较差异有统计学意义(Control组,1.47∀0.18;1.5%异氟烷组,1.19∀0.13;2.0%异氟烷组,
4、1.13∀0.12;P<0.01)。结论:吸入1.5%或更高浓度的异氟烷8h,可降低大鼠肺组织SPA的基因表达和蛋白合成,并可能影响其分泌和再摄取。[关键词]异氟醚;肺表面活性剂;基因表达[中图分类号]R6141[文献标识码]A[文章编号]1671167X(2006)04040205EffectsofisofluraneonlungsurfactantproteinAinratsLIZhihong,YANGBaxian,ANHaiyan(DepartmentofAnesthesiology,Peki
5、ngUniversityPeople#sHospita,lBeijing100044,China)ABSTRACTObjective:ToexaminetheeffectsofdifferentconcentrationsofIsofluraneonthelevelofsurfactantproteinA(SPA)andtheexpressionofSPAmRNAinthelungofrats.Methods:ThirtytwomaleWistarratswererandomlydividedinto4grou
6、ps:controlgroupreceived40%(volumefraction)O2inhalation(40%O2,n=8);0.7%isofluranegroup(n=8),1.5%isofluranegroup(n=8).Theyand2.0%isofluranegroup(n=8)Theyweretreatedwith40%O2with0.7%,1.5%and2.0%isofluranerespectively.Ineachgroup,theratsinhaledexperimentalgasfor8h
7、ours,andthenwereputtodeathimmediately.Morphologicalchangesoftype!pneumocyteswereobservedbytransmissionelectronmicroscopy.Surfactantproteincontentinbronchoalveolarlavagefluid(BALF)wasmeasuredwithWesternBlotting.ThelevelofintracellularSPAwasexaminedwithimmunoh
8、istochemistry(IHC).ToassesstherelativelevelsofSPAmRNAinlungtissue,reversetranscriptasepolymerasechainreaction(RTPCR)wasusedwiththecoamplificationofthe∃housekeeping%geneG
