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1、ChineseJournalofNewDrugs2010,19(24)实验研究谷胱甘肽复方注射液治疗猪血清致大鼠肝纤维化的药效研究梁跃东,李伟,曾经章,危敏,程明亮,吴君(贵阳医学院感染病学教研室,贵阳550004)[摘要]目的:研究谷胱甘肽复方注射液(CGII)治疗猪血清致大鼠免疫性肝纤维化的药效作用。方法:建立猪血清诱导免疫性肝纤维化模型,全自动生化仪检测血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白蛋白(A)及球蛋白(G)水平;酶免法检测血清中透明质酸(HA)的含量;称取肝脏湿
2、重,计算肝脏指数;消化法检测肝组织羟脯氨酸(Hyp)含量;苏木素-伊红(HE)及Masson染色处理肝脏组织切片;光镜观察病理学改变。结果:在猪血清诱导的大鼠肝纤维化模型中,所有剂量水平的CGII(2.7,5.4,-1-1108mgkg,im)能显著降低大鼠血清ALT,AST,A/G值和HA水平;中、高剂量CGII(5.4,10.8mgkg,-1im)能显著降低大鼠肝组织Hyp含量,明显改善肝脏病理组织状况;高剂量CGII(10.8mgkg,im)能显著降低大鼠肝脏指数。结论:CGII对猪血清致大鼠肝纤维化有治
3、疗作用。[关键词]谷胱甘肽复方注射液;猪血清;肝纤维化;药效[中图分类号]R965.1;R975.5[文献标志码]A[文章编号]1003-3734(2010)24-2323-05TherapeuticeffectofcompoundglutathioneinosineinjectiononimmunologicallyinducedliverfibrosisinratsLIANGYue-dong,LIWe,iZENGJing-zhang,WEIMin,CHENGMing-liang,WUJun(TheInfect
4、iveDiseaseStaffroomofGuiyangMedicalCollege,Guiyang550004,China)[Abstract]Objective:Tostudythetherapeuticeffectofcompoundglutathioneinosineinjection(CGII)onliverfibrosisimmunologicallyinducedbyporcineseruminrats.Methods:Theliverfibrosiswasinducedbyintrap-eritone
5、alinjectionofporcineserum.Theserumactivitiesofalanineaminotransferase(ALT),aspartateaminotrans-ferase(AST),albumin(A)andglobulin(G)wereassayedbyautomaticbiochemistrydetectioninstrumen.tTheserumhaluronicacid(HA),ahepaticfibrosisindex,wasdeterminedbyELISAmethod.Li
6、verindexwascalcula-ted;hydroxyproline(Hyp)contentinlivertissuewasassayedbyalkalinehydrolysiscolorimetry.Thehepatichisto-logicalchangeswerealsoobservedbyopticalmicroscopyafterHEandMassonstaining.Results:Inratswithliver-1fibrosis,CGII(2.7,5.4,10.8mgkg,im)signific
7、antlydecreasedtheserumlevelsofAST,ALT,A/Gand-1HA;CGII(5.4,10.8mgkg,im)obviouslyimprovedthepathologicallesionofliverfibrosisanddecreased-1Hypcontentsinliver;CGII(10.8mgkg,im)alsodecreasedliverindex.Conclusion:CGIIhasatherapeuticeffectonhepaticfibrosisimmunologi
8、callyinducedbyporcineseruminrats.[Keywords]compoundglutathioneinosineinjection;porcineserum;liverfibrosis;therapeuticeffect[基金项目]香港兆科药业科研基金项目[作者简介]梁跃东,男,副教授,在读博士。[
