tgf-β_1对肾间质成纤维细胞sdf-1表达的影响

《tgf-β_1对肾间质成纤维细胞sdf-1表达的影响》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、四川大学学撰医学E版diJSichuanUnivMedSciEdi)2。。o。1。o；，4l(3)：_453—457UnivTGF—P1对肾间质成纤维细胞SDF一1表达的影响张和平，谢席胜，张蕾，樊均明1,2A1．四川大学华西医院肾脏内科(成都610041)；2．四川大学华西医院人类疾病生物治疗国家重点实验室(成都610041)【摘要】目的探讨大鼠肾间质成纤维细胞(NRK49F)基质细胞衍生因子一1(stromalcellderivedfactor一1，SDF-1)的表达及转化生长因子一B1(trans

2、forminggrowthfactor-13l，TGF-131)对其表达的影响。方法采用RT-PCR和Westernblot及免疫组化方法检测NRK49F细胞SDF-1表达及不同浓度、不同时间的TGF-~I刺激对SDF-I表达的影响。结果正常NRK49F细胞低表达SDF-1mRNA，TGF-~-呈时间依赖性增加其表达，在24h表达最高，为刺激前(2．924-+"0．235)倍，差异有统计学意义(P

3、差异有统计学意义(P

4、化的发生发展。【关键词】转化生长因子一口z基质细胞衍生因子一l成纤维细胞【中图分类号】R692．6TansformingGrowthTGF-I~MediatestheExpressionofStromalCellDerivedFactor-1inKidneyInterstitialFibroblastCellsinRatsZHANGHe-ping，XIEXi—sheng，ZHANGLei，FANJun—ming’．1．DepartmentofNephrology，WestChinaHosptial，Si

5、chuanUniversity，Chengdu610041，China；2．StateKeyLaboratoryofBiotherapyofHumanDiease，WestChinaHospital，SichuanUniversity，Chengdu610041，China△Correspondingauthor，E-mail：junmingfan2007@yahoo．com．cn[Abstract]ObjectiveTostudytheexpressionpatternofSDF-1intheNRK4

6、9FcellsandtheroleofTGF-~,inmediatingtheexpressionofSDF-1．MethodsTheSDF-1mRNAandproteinexpressionintheNRK49FcellswithorwithoutstimulatingbyTGF-181wasassayedwithRT-PCRorWesternblotorImmunohistochemistry．ResultsTheSDF-1mRNAexpressionstimulatedbyTGF-~,appear

7、edinatime-dependentmanner．Thepeakvalueappearedat24hoursandwas(2．924士0．235)timesashighastheinitia1leve1．Thedose-coursestudiessuggestedthatTGF-~lstimulationresultedinmarkedpromotionofSDF-1mRNAexpression，whichpeakedattheconcentrationof5ng／mL．Atthisconcentra

8、tion，theexpressionofSDF-1mRNAwas(2．113士0．314)timesashighasthatofthecontro1．CorrespondingwiththepatternofmRNAexpressionofSDF-1，proteinexpressionofSDF-1wasobservedinNRK49Fcells．Atime-dependentmannerwasalsoobservedintheprotei