骨代谢标志物(ntx)与大鼠骨转移疼痛的关系研究

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1、分类号学号D201078362学校代码10487密级博士学位论文骨代谢标志物（NTx）与大鼠骨转移疼痛的关系研究学位申请人：桂琦学科专业：肿瘤学指导教师：于世英教授答辩日期：2013年5月AdissertationsubmittedtoHuazhongUniversityofScienceandTechnologyfortheDegreeofDoctorofMedicineThecorrelationbetweenbonemetabolicmarker(NTx)andratbonecancerpainM.D.Candidate:QiGuiMajor:OncologySuperv

2、isor:Prof.ShiyingYuHuazhongUniversityofScience&TechnologyWuhan430074,P.R.ChinaMay,2013独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校

3、有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。保密□，在_____年解密后适用本授权书。本论文属于不保密□。（请在以上方框内打“√”）学位论文作者签名：指导教师签名：日期：年月日日期：年月日目录英文缩略词表························································································1中文摘要··············

4、····················································································2英文摘要··································································································6第一部分不同骨肿瘤疼痛动物模型拟合临床特征的研究················10第二部分骨转移疼痛新的大鼠模型：在大鼠股骨第三转子处种植大鼠乳腺癌细胞···························

5、······························21第三部分骨代谢标记物NTx与大鼠骨转移疼痛的关系研究············39全文总结································································································59综述骨代谢标记物与肿瘤骨转移·······················································60附录1攻读学位期间撰写与发表的研究论文····························

6、·········76附录2致谢···························································································77华中科技大学博士学位论文缩略词及中英文对照缩略词英文全称中文全称c-fosProto-oncogenec-fos原癌基因c-fosTRPV1Transientreceptorpotentialvanilloid瞬时受体电位香草素1SPSubstancePP物质ASIC3Acid-sensingionchannel3酸敏感离子通道3GAPDHGlyceraldehy

7、de-3-phosphatedehydrogenas甘油醛-3-磷酸脱氢酶EDTAEthyleneDiamineTetraaceticAcid乙二胺四乙酸SREsSkeletal-relatedevents骨相关事件TRAPTartrate-resistantacidphosphatase抗酒石酸酸性磷酸酶RT-PCRReal-timepolymerasechainreaction实时定量聚合酶链式反应BCEBonecollagenequivalents骨胶原当量NTxTypeIcollag