杂化材料和亲水共聚树脂材料制备及在管尖固相萃取中的应用研究

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1、ClassifiedIndex:CODE:10075U.D.C.:NO:20121415ADissertationfortheDegreeofM.SciencePreparationofHybridMaterialandHydrophilicCopolymerResinMaterialanditsApplicationinPipette-TipSolidPhaseExtractionCandidate:WangMingyuSupervisor:Prof.YanHongyuanAcademicDegreeAppliedfor:MasterofScienceSpecialty:Pharmace

2、uticalAnalysisUniversity:HebeiUniversityDateofOralExamination:June,2015摘要摘要针对目前生命科学领域分离分析的难点和热点，立足于复杂样品中痕量组分的前处理技术，固相萃取自其问世以来，已成为迄今为止应用最为广泛和成熟的样品前处理技术之一，且在生物、食品、环境、医药等多领域占据了重要地位。由此演化而来的小型化固相萃取技术，能够在保持萃取效率不降低的情况下，进一步简化前处理操作，缩短分析时间。本文主要开发两种新型吸附剂，并将其应用于管尖固相萃取技术，实现蔬菜水果中痕量目标物的快速分离和富集。第一章，对小型化固相萃取技术及吸附剂

3、材料的研究进展进行总结，并重点介绍了有机-无机杂化材料和亲水共聚树脂材料。第二章，通过溶胶-凝胶聚合过程制备了有机-无机杂化材料，建立了溶胶-凝胶杂化吸附剂-管尖固相萃取-高效液相色谱法，成功应用于黄瓜中三嗪类除草剂的快速分离和检测。第三章，采用水溶性共聚单体和印迹模板，制备出了亲水印迹共聚树脂，应用于管尖固相萃取-高效液相色谱法，实现了椰汁样品中痕量植物激素简单、快速的分离分析。关键词固相萃取管尖固相萃取有机-无机杂化材料亲水共聚树脂材料IAbstractAbstractAimingatthesubsistentproblemsoflifesciencesandpretreatmentt

4、echnologiesoftracecomponentsincomplexsamples,solid-phaseextractionhasbecomeoneofthemostpopulartechniquesinbiologicalchemistry,foodanalysis,environmentalmonitoring,andclinicalpharmacy.Theminiaturizedsolid-phaseextractionisthederivativeofsolid-phaseextraction.Itcouldeffectivelyreduceoperationandshor

5、tenanalysistimewithhighextractionefficiency.Inthispaper,twonewadsorbentsweresynthesizedandusedinpipette-tipsolidphaseextractiontorapidlyseparateandconcentratetraceanalytesinfruitsandvegetables.Chapterone,thispartsummarizedtheresearchofminiaturizedsolid-phaseextractiontechniquesandtheiradsorbents,a

6、ndprimarilyfocusedonorganic-inorganichybridmaterialsandhydrophiliccopolymerresinmaterials.Chaptertwo,thispartsynthesizedanorganic-inorganichybridmaterialthroughsol-gelpolymerizationanddevelopedapipette-tipsolid-phaseextraction-highperformanceliquidchromatographymethod.Thepresentedmethodwassuccessf

7、ullyusedtoseparateanddetecttriazineherbicidesincucumber.Chapterthree,ahydrophilicmolecularlyimprintedcopolymerresinwassynthesizedusinghydrophilicmonomersandahydrophilictemplate.Itwasappliedtothepipette-tipsolidph