基于hk-2细胞评价药物肾毒性体外方法的建立

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1、致谢衷心感谢伟大的社会主义祖国，祖国的繁荣、昌盛和“科教兴国”的基本国策给予我们攻读高级学位的机会；感谢天津市和天津中医药大学为我们建立了学习实践和科学研究的平台，提供了丰富的教学资源；感谢国家重大新药创制专项(项目编号：2012ZX09505)对本论文所提供的支持。向我最尊敬的导师张宗鹏致以最崇高的谢意！导师渊博的专业知识，严谨的治学态度，精益求精的工作作风，诲人不倦的高尚师德，严以律己、宽以待人的崇高风范，朴实无华、平易近人的人格魅力对我影响深远。不仅使我树立了远大的学术目标、掌握了基本的研究方法，还使我明白了许多

2、待人接物与为人处世的道理。感谢张金晓老师，无论是论文的选题，还是试验设计、结果的分析，直至论文的撰写，都倾注了大量的心血。张老师严谨的办事风格、仔细认真的态度、敏锐的科研思路、坚韧不拔的拼搏精神是我三年求学生涯中获得的一笔宝贵的人生财富。在此，我向她奉上深深的谢意，感谢她的无私奉献。感谢柴振海、程晓翔、姜凌、张建军等老师在实验过程和生活中给予我的大力帮助。同时感谢李黎、杨佳梅、冯玥、郭舜等对我的帮助。深深地感谢父母及家人对我一如既往的支持与鼓励，让我能够勇敢地面对生活中的任何困难与挑战，并顺利完成学业。最后，对精心审阅

3、本论文的各位老师致以最诚挚的谢意！1天津中医药大学硕士学位论文目录中文摘要···································································································1ABSTRACT·······························································································4英文缩略表················

4、················································································7综述·········································································································8前言·······································································

5、································21研究内容一HK-2细胞用于药物肾毒性体外研究的可行性评价···························23第一部分HK-2及L-02细胞的培养及生长曲线的建立·······································231材料与方法····························································································232结果·

6、····································································································273小结·····································································································30第二部分HK-2及L-02细胞用于药物毒性器官选择性的研究·························

7、·····311材料与方法····························································································312结果·····································································································363小结··············································

8、·······················································44研究内容二基于HK-2细胞评价药物肾毒性体外检测指标的确立·······················46第一部分药物对HK-2细胞的毒效应及其相应毒性指标的检测···························